2014
Exploring the cystatins of helminths
ILGOVÁ, Jana, Břetislav KOUDELA, Milan GELNAR a Martin KAŠNÝZákladní údaje
Originální název
Exploring the cystatins of helminths
Autoři
ILGOVÁ, Jana (703 Slovensko, garant, domácí), Břetislav KOUDELA (203 Česká republika), Milan GELNAR (203 Česká republika, domácí) a Martin KAŠNÝ (203 Česká republika, domácí)
Vydání
V4 Parasitological meeting, 2014
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Slovensko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/14:00073668
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-80-968473-7-2
Klíčová slova anglicky
Eudiplozoon nipponicum; Hymenolepis diminuta; Fascioloides magna; Trichinella spiralis; cystatin; inhibitor; PCR RACE; coding sequence
Změněno: 5. 6. 2014 12:00, Mgr. et Mgr. Jana Ilgová, Ph.D.
Anotace
V originále
Parasitic organisms release numerous types of molecules essential for their survival. Cystatins - cysteine peptidase inhibitors (CPI) were determined as important protein molecules which participate in the processes in the host-parasite interface. Their effect can influence the host immune response, particularly the processing and presentation of antigens, production of nitric oxide, etc. The aim of our study is to expand the information about cystatins produced by helminths and to reveal their functions in four selected parasitic model species representing the major groups of helminths. We used bioinformatic tools to analyze the transcriptomic and/or genomic data of Eudiplozoon nipponicum (Monogenea), Hymenolepis diminuta (Cestoda), Fascioloides magna (Trematoda) and Trichinella spiralis (Nematoda) and we searched for the presence of conserved cystatin domains. The selected genes of CPI of diverse families - stefins, cystatins and kininogens were amplified, cloned and sequenced and significant differences in their nucleotide/amino acid primary structure were revealed. The production of recombinant forms of CPI is in process in order to characterize their structures and functions.
Návaznosti
GAP506/12/1258, projekt VaV |
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GBP505/12/G112, projekt VaV |
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MUNI/A/0888/2013, interní kód MU |
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