Compromised telomere maintenance in hypomethylated Arabidopsis
thaliana plants

J 2014

Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants

OGROCKÁ, Anna; Pavla POLANSKÁ; Eva MAJEROVÁ; Zlatko JANEBA; Jiří FAJKUS et al.

Základní údaje

Originální název

Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants

Autoři

OGROCKÁ, Anna; Pavla POLANSKÁ; Eva MAJEROVÁ; Zlatko JANEBA; Jiří FAJKUS a Miloslava FOJTOVÁ

Vydání

Nucleic Acids Research, OXFORD, Oxford University Press, 2014, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.112

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/14:00073694

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

DNA METHYLATION; CYTOSINE METHYLATION; MAMMALIAN TELOMERES; EPIGENETIC INHERITANCE; CHROMOSOME ENDS; BINDING PROTEIN; NONCODING RNA; TOBACCO CELLS; GENOME; LENGTH

Štítky

kontrola MP, OA, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2014 15:38, Martina Prášilová

Anotace

V originále

Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are important for the maintenance of genomic stability. Telomeres were considered as typical heterochromatic regions, but in light of recent results, this view should be reconsidered. Asymmetrically located cytosines in plant telomeric DNA repeats may be substrates for a DNA methyltransferase enzyme and indeed, it was shown that these repeats are methylated. Here, we analyse the methylation of telomeric cytosines and the length of telomeres in Arabidopsis thaliana methylation mutants (met 1-3 and ddm 1-8), and in their wild-type siblings that were germinated in the presence of hypomethylation drugs. Our results show that cytosine methylation in telomeric repeats depends on the activity of MET1 and DDM1 enzymes. Significantly shortened telomeres occur in later generations of methylation mutants as well as in plants germinated in the presence of hypomethylation drugs, and this phenotype is stably transmitted to the next plant generation. A possible role of compromised in vivo telomerase action in the observed telomere shortening is hypothesized based on telomere analysis of hypomethylated telomerase knockout plants. Results are discussed in connection with previous data in this field obtained using different model systems.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GAP501/11/0596, projekt VaV

Název: Epigenetické mechanismy regulace rostlinných telomer (Akronym: epigenetika telomer)

Investor: Grantová agentura ČR, Epigenetické mechanismy regulace rostlinných telomer

Přiložené soubory

ZVV_2014_058_1186309_Compromised_telomere.pdf

Požádat o autorskou verzi souboru

Citovat

OGROCKÁ, Anna; Pavla POLANSKÁ; Eva MAJEROVÁ; Zlatko JANEBA; Jiří FAJKUS a Miloslava FOJTOVÁ. Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants. Nucleic Acids Research. OXFORD: Oxford University Press, 2014, roč. 42, č. 5, s. 2919-2931. ISSN 0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkt1285.

@article{1186309,
   author = {Ogrocká, Anna and Polanská, Pavla and Majerová, Eva and Janeba, Zlatko and Fajkus, Jiří and Fojtová, Miloslava},
   article_location = {OXFORD},
   article_number = {5},
   doi = {https://doi.org/10.1093/nar/gkt1285},
   keywords = {DNA METHYLATION; CYTOSINE METHYLATION; MAMMALIAN TELOMERES; EPIGENETIC INHERITANCE; CHROMOSOME ENDS; BINDING PROTEIN; NONCODING RNA; TOBACCO CELLS; GENOME; LENGTH},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants},
   url = {http://nar.oxfordjournals.org/content/42/5/2919.long},
   volume = {42},
   year = {2014}
}

TY  - JOUR
ID  - 1186309
AU  - Ogrocká, Anna - Polanská, Pavla - Majerová, Eva - Janeba, Zlatko - Fajkus, Jiří - Fojtová, Miloslava
PY  - 2014
TI  - Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants
JF  - Nucleic Acids Research
VL  - 42
IS  - 5
SP  - 2919-2931
EP  - 2919-2931
PB  - Oxford University Press
SN  - 03051048
KW  - DNA METHYLATION
KW  - CYTOSINE METHYLATION
KW  - MAMMALIAN TELOMERES
KW  - EPIGENETIC INHERITANCE
KW  - CHROMOSOME ENDS
KW  - BINDING PROTEIN
KW  - NONCODING RNA
KW  - TOBACCO CELLS
KW  - GENOME
KW  - LENGTH
UR  - http://nar.oxfordjournals.org/content/42/5/2919.long
L2  - http://nar.oxfordjournals.org/content/42/5/2919.long
N2  - Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are important for the maintenance of genomic stability. Telomeres were considered as typical heterochromatic regions, but in light of recent results, this view should be reconsidered. Asymmetrically located cytosines in plant telomeric DNA repeats may be substrates for a DNA methyltransferase enzyme and indeed, it was shown that these repeats are methylated. Here, we analyse the methylation of telomeric cytosines and the length of telomeres in Arabidopsis thaliana methylation mutants (met 1-3 and ddm 1-8), and in their wild-type siblings that were germinated in the presence of hypomethylation drugs. Our results show that cytosine methylation in telomeric repeats depends on the activity of MET1 and DDM1 enzymes. Significantly shortened telomeres occur in later generations of methylation mutants as well as in plants germinated in the presence of hypomethylation drugs, and this phenotype is stably transmitted to the next plant generation. A possible role of compromised in vivo telomerase action in the observed telomere shortening is hypothesized based on telomere analysis of hypomethylated telomerase knockout plants. Results are discussed in connection with previous data in this field obtained using different model systems.
ER  -

OGROCKÁ, Anna; Pavla POLANSKÁ; Eva MAJEROVÁ; Zlatko JANEBA; Jiří FAJKUS a Miloslava FOJTOVÁ. Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants. \textit{Nucleic Acids Research}. OXFORD: Oxford University Press, 2014, roč.~42, č.~5, s.~2919-2931. ISSN~0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkt1285.

Přehled o publikaci