HAFIDH, Said, David POTĚŠIL, Jan FÍLA, Jana FECÍKOVÁ, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL a David HONYS. In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. BIOCHEMICAL SOCIETY TRANSACTIONS. LONDON: PORTLAND PRESS LTD, 2014, roč. 42, April, s. 388-394. ISSN 0300-5127. Dostupné z: https://dx.doi.org/10.1042/BST20130204. |
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@article{1186360, author = {Hafidh, Said and Potěšil, David and Fíla, Jan and Fecíková, Jana and Čapková, Věra and Zdráhal, Zbyněk and Honys, David}, article_location = {LONDON}, article_number = {April}, doi = {http://dx.doi.org/10.1042/BST20130204}, keywords = {GPI-ANCHORED PROTEIN; PLANT SECRETOME; ARABIDOPSIS; GROWTH; GUIDANCE; GERMINATION; ATTRACTANT; COMPONENTS; MAIZE; CELL}, language = {eng}, issn = {0300-5127}, journal = {BIOCHEMICAL SOCIETY TRANSACTIONS}, title = {In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception}, url = {http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm}, volume = {42}, year = {2014} }
TY - JOUR ID - 1186360 AU - Hafidh, Said - Potěšil, David - Fíla, Jan - Fecíková, Jana - Čapková, Věra - Zdráhal, Zbyněk - Honys, David PY - 2014 TI - In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception JF - BIOCHEMICAL SOCIETY TRANSACTIONS VL - 42 IS - April SP - 388-394 EP - 388-394 PB - PORTLAND PRESS LTD SN - 03005127 KW - GPI-ANCHORED PROTEIN KW - PLANT SECRETOME KW - ARABIDOPSIS KW - GROWTH KW - GUIDANCE KW - GERMINATION KW - ATTRACTANT KW - COMPONENTS KW - MAIZE KW - CELL UR - http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm L2 - http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm N2 - The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome. ER -
HAFIDH, Said, David POTĚŠIL, Jan FÍLA, Jana FECÍKOVÁ, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL a David HONYS. In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. \textit{BIOCHEMICAL SOCIETY TRANSACTIONS}. LONDON: PORTLAND PRESS LTD, 2014, roč.~42, April, s.~388-394. ISSN~0300-5127. Dostupné z: https://dx.doi.org/10.1042/BST20130204.
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