Determination of common urine substances as an assay for
improving prostate carcinoma diagnostics

J 2014

Determination of common urine substances as an assay for improving prostate carcinoma diagnostics

HEGER, Zbynek, Natalia Vladimirovna CERNEI, Jaromír GUMULEC, Michal MASAŘÍK, Tomas ECKSCHLAGER et. al.

Základní údaje

Originální název

Determination of common urine substances as an assay for improving prostate carcinoma diagnostics

Autoři

HEGER, Zbynek (203 Česká republika), Natalia Vladimirovna CERNEI (498 Moldavsko), Jaromír GUMULEC (203 Česká republika, domácí), Michal MASAŘÍK (203 Česká republika, garant, domácí), Tomas ECKSCHLAGER (203 Česká republika), Roman HRABEC (203 Česká republika, domácí), Ondřej ZÍTKA (203 Česká republika), Vojtěch ADAM (203 Česká republika) a René KIZEK (203 Česká republika)

Vydání

Oncology Reports, ATHENS, SPANDIDOS PUBL LTD, 2014, 1021-335X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.301

Kód RIV

RIV/00216224:14110/14:00075838

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3892/or.2014.3054

UT WoS

000334345600046

Klíčová slova anglicky

PSA; immunoenzymometric assay; ion-exchange liquid chromatography; sarcosine; spectrophotometry; proline

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 11. 2014 09:29, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Recently, interest in the identification of non-invasive markers for prostate carcinoma detectable in the urine of patients has increased. In this study, we monitored the abundance of potential non-invasive markers of prostate carcinoma such as amino acid sarcosine, involved in the metabolism of amino acids and methylation processes, ongoing during the progression of prostate carcinoma. In addition, other potential prostate tumor markers were studied. The most significant markers, prostate-specific antigen (PSA) and free PSA (fPSA), already used in clinical diagnosis, were analyzed using an immunoenzymometric assay. Whole amino acid profiles were also determined to evaluate the status of amino acids in patient urine samples and to elucidate the possibility of their utilization for prostate carcinoma diagnosis. To obtain the maximum amount of information, the biochemical parameters were determined using various spectrophotometric methods. All results were subjected to statistical processing for revealing different correlations between the studied parameters. We observed alterations in most of the analyzed substances. Based on the results obtained, we concluded that the specificity of prostate carcinoma diagnosis could be improved by determination of common urine metabolites, since we compiled a set of tests, including the analysis of sarcosine, proline, PSA and uric acid in the urine. These metabolites were not observed in the urine obtained from healthy subjects, while their levels were elevated in all patients suffering from prostate carcinoma.

Citovat

HEGER, Zbynek, Natalia Vladimirovna CERNEI, Jaromír GUMULEC, Michal MASAŘÍK, Tomas ECKSCHLAGER, Roman HRABEC, Ondřej ZÍTKA, Vojtěch ADAM a René KIZEK. Determination of common urine substances as an assay for improving prostate carcinoma diagnostics. Oncology Reports. ATHENS: SPANDIDOS PUBL LTD, 2014, roč. 31, č. 4, s. 1846-1854. ISSN 1021-335X. Dostupné z: https://dx.doi.org/10.3892/or.2014.3054.

@article{1187241,
   author = {Heger, Zbynek and Cernei, Natalia Vladimirovna and Gumulec, Jaromír and Masařík, Michal and Eckschlager, Tomas and Hrabec, Roman and Zítka, Ondřej and Adam, Vojtěch and Kizek, René},
   article_location = {ATHENS},
   article_number = {4},
   doi = {http://dx.doi.org/10.3892/or.2014.3054},
   keywords = {PSA; immunoenzymometric assay; ion-exchange liquid chromatography; sarcosine; spectrophotometry; proline},
   language = {eng},
   issn = {1021-335X},
   journal = {Oncology Reports},
   title = {Determination of common urine substances as an assay for improving prostate carcinoma diagnostics},
   volume = {31},
   year = {2014}
}

TY  - JOUR
ID  - 1187241
AU  - Heger, Zbynek - Cernei, Natalia Vladimirovna - Gumulec, Jaromír - Masařík, Michal - Eckschlager, Tomas - Hrabec, Roman - Zítka, Ondřej - Adam, Vojtěch - Kizek, René
PY  - 2014
TI  - Determination of common urine substances as an assay for improving prostate carcinoma diagnostics
JF  - Oncology Reports
VL  - 31
IS  - 4
SP  - 1846-1854
EP  - 1846-1854
PB  - SPANDIDOS PUBL LTD
SN  - 1021335X
KW  - PSA
KW  - immunoenzymometric assay
KW  - ion-exchange liquid chromatography
KW  - sarcosine
KW  - spectrophotometry
KW  - proline
N2  - Recently, interest in the identification of non-invasive markers for prostate carcinoma detectable in the urine of patients has increased. In this study, we monitored the abundance of potential non-invasive markers of prostate carcinoma such as amino acid sarcosine, involved in the metabolism of amino acids and methylation processes, ongoing during the progression of prostate carcinoma. In addition, other potential prostate tumor markers were studied. The most significant markers, prostate-specific antigen (PSA) and free PSA (fPSA), already used in clinical diagnosis, were analyzed using an immunoenzymometric assay. Whole amino acid profiles were also determined to evaluate the status of amino acids in patient urine samples and to elucidate the possibility of their utilization for prostate carcinoma diagnosis. To obtain the maximum amount of information, the biochemical parameters were determined using various spectrophotometric methods. All results were subjected to statistical processing for revealing different correlations between the studied parameters. We observed alterations in most of the analyzed substances. Based on the results obtained, we concluded that the specificity of prostate carcinoma diagnosis could be improved by determination of common urine metabolites, since we compiled a set of tests, including the analysis of sarcosine, proline, PSA and uric acid in the urine. These metabolites were not observed in the urine obtained from healthy subjects, while their levels were elevated in all patients suffering from prostate carcinoma.
ER  -

HEGER, Zbynek, Natalia Vladimirovna CERNEI, Jaromír GUMULEC, Michal MASAŘÍK, Tomas ECKSCHLAGER, Roman HRABEC, Ondřej ZÍTKA, Vojtěch ADAM a René KIZEK. Determination of common urine substances as an assay for improving prostate carcinoma diagnostics. \textit{Oncology Reports}. ATHENS: SPANDIDOS PUBL LTD, 2014, roč.~31, č.~4, s.~1846-1854. ISSN~1021-335X. Dostupné z: https://dx.doi.org/10.3892/or.2014.3054.

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