2014
Biosensing of purine derivatives using a pencil graphite electrode modified by copper: a promising tool in biomedicine
TRNKOVÁ, Libuše, Rudolf NAVRÁTIL a Vimal Kumar SHARMAZákladní údaje
Originální název
Biosensing of purine derivatives using a pencil graphite electrode modified by copper: a promising tool in biomedicine
Název česky
Biosnímání purinových derivátů s využitím pentelkové grafitové elektrody modifikované mědí: slibný nástroj v biomedicíně
Autoři
TRNKOVÁ, Libuše, Rudolf NAVRÁTIL a Vimal Kumar SHARMA
Vydání
Malmo, 15th International Conference on Electroanalysis, od s. 228-228, 1 s. 2014
Nakladatel
neuveden
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10403 Physical chemistry
Stát vydavatele
Švédsko
Utajení
není předmětem státního či obchodního tajemství
Forma vydání
tištěná verze "print"
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
nanočástice mědi; pentelková grafitová elektroda;kompex Cu(I)-purin; lineární sweep voltametrie; eliminační voltametrie
Klíčová slova anglicky
copper nanoparticles; pencil graphite electrode; Cu(I)-purine complex; linear sweep voltammetry; elimination voltammetry
Štítky
Změněno: 17. 5. 2018 10:58, Ing. Nicole Zrilić
Anotace
V originále
Purine derivatives are important substances which can be found in blood, serum, urone and other physiological liquids as product of human biochemical processes known under the term purine catabolism. Here we suggest a simple, sensitive, easily accessible and very cheap tool for their electrochemical analysis - a pencil graphite elctrode (PeGE) modified by copper ions was performed by the reduction of Cu(II) from solution or by the oxidation of Cu(0) from nanoparticles immobilized on the electrode surface. Purine derivatives react with Cu(I) to form Cu(I)-puirne complexes which remain for s certain timr at the electrode surface. This time is used for the linear sweep voltammetric (LSV) experiment in an adsorptive stripping mode. The application of elimination voltammetry with linear scan (EVLS), which provides a special signal for a totally adsorptive electroactive species, allows increasing substantially the sensitivity evaluated by LOD from micromolar to units of nanomolar concentrations and separating overlapped purine signals. Moreover, the sensing can employ not only oxidation signals of purine derivatives but also oxidation signals of the corresponding copper complexes. These complexes were identified by means of titration of electrochemicallly produced cuprous ions by purine derivatives. The detection limits achieved by this approach are three orders of magnitude lower than those found with UV absorbance detection. As the detection does not require oxygen -free environment, our PeGE modified by monovalent copper may serve as a suitable electrochemical sensor for purines also in biomedicine.
Návaznosti
CZ.1.05/1.1.00/02.0068, interní kód MU |
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LH13053, projekt VaV |
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MUNI/A/0972/2013, interní kód MU |
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