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@inproceedings{1187924,
author = {Trnková, Libuše and Navrátil, Rudolf and Sharma, Vimal Kumar},
address = {Malmo},
booktitle = {15th International Conference on Electroanalysis},
keywords = {copper nanoparticles; pencil graphite electrode; Cu(I)-purine complex; linear sweep voltammetry; elimination voltammetry},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Malmo},
note = {Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+},
pages = {228-228},
publisher = {neuveden},
title = {Biosensing of purine derivatives using a pencil graphite electrode modified by copper: a promising tool in biomedicine},
year = {2014}
}
TY - JOUR
ID - 1187924
AU - Trnková, Libuše - Navrátil, Rudolf - Sharma, Vimal Kumar
PY - 2014
TI - Biosensing of purine derivatives using a pencil graphite electrode modified by copper: a promising tool in biomedicine
PB - neuveden
CY - Malmo
N1 - Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+
KW - copper nanoparticles
KW - pencil graphite electrode
KW - Cu(I)-purine complex
KW - linear sweep voltammetry
KW - elimination voltammetry
N2 - Purine derivatives are important substances which can be found in blood, serum, urone and other physiological liquids as product of human biochemical processes known under the term purine catabolism. Here we suggest a simple, sensitive, easily accessible and very cheap tool for their electrochemical analysis - a pencil graphite elctrode (PeGE) modified by copper ions was performed by the reduction of Cu(II) from solution or by the oxidation of Cu(0) from nanoparticles immobilized on the electrode surface. Purine derivatives react with Cu(I) to form Cu(I)-puirne complexes which remain for s certain timr at the electrode surface. This time is used for the linear sweep voltammetric (LSV) experiment in an adsorptive stripping mode. The application of elimination voltammetry with linear scan (EVLS), which provides a special signal for a totally adsorptive electroactive species, allows increasing substantially the sensitivity evaluated by LOD from micromolar to units of nanomolar concentrations and separating overlapped purine signals. Moreover, the sensing can employ not only oxidation signals of purine derivatives but also oxidation signals of the corresponding copper complexes. These complexes were identified by means of titration of electrochemicallly produced cuprous ions by purine derivatives. The detection limits achieved by this approach are three orders of magnitude lower than those found with UV absorbance detection. As the detection does not require oxygen -free environment, our PeGE modified by monovalent copper may serve as a suitable electrochemical sensor for purines also in biomedicine.
ER -
TRNKOVÁ, Libuše, Rudolf NAVRÁTIL a Vimal Kumar SHARMA. Biosensing of purine derivatives using a pencil graphite electrode modified by copper: a promising tool in biomedicine. In \textit{15th International Conference on Electroanalysis}. Malmo: neuveden, 2014, s.~228-228.
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