| SEDLAŘÍKOVÁ, Lenka, Lenka BEŠŠE, Fedor KRYUKOV, Jana PELCOVA, Zdeněk ADAM, Luděk POUR, Roman HÁJEK and Sabina ŠEVČÍKOVÁ. Detection of tumor-specific marker for minimal residual disease in multiple myeloma patients. Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic. Olomouc: Palacký University, 2015, vol. 159, No 4, p. 554-561. ISSN 1213-8118. Available from: https://dx.doi.org/10.5507/bp.2014.035. |
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@article{1188724,
author = {Sedlaříková, Lenka and Bešše, Lenka and Kryukov, Fedor and Pelcova, Jana and Adam, Zdeněk and Pour, Luděk and Hájek, Roman and Ševčíková, Sabina},
article_location = {Olomouc},
article_number = {4},
doi = {http://dx.doi.org/10.5507/bp.2014.035},
keywords = {multiple myeloma; minimal residual disease; tumor-specific marker; ASO PCR; RQ-PCR; IgH gene rearrangement},
language = {eng},
issn = {1213-8118},
journal = {Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic},
note = {http://biomed.papers.upol.cz/},
title = {Detection of tumor-specific marker for minimal residual disease in multiple myeloma patients},
volume = {159},
year = {2015}
}
TY - JOUR
ID - 1188724
AU - Sedlaříková, Lenka - Bešše, Lenka - Kryukov, Fedor - Pelcova, Jana - Adam, Zdeněk - Pour, Luděk - Hájek, Roman - Ševčíková, Sabina
PY - 2015
TI - Detection of tumor-specific marker for minimal residual disease in multiple myeloma patients
JF - Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic
VL - 159
IS - 4
SP - 554-561
EP - 554-561
PB - Palacký University
SN - 12138118
N1 - http://biomed.papers.upol.cz/
KW - multiple myeloma
KW - minimal residual disease
KW - tumor-specific marker
KW - ASO PCR
KW - RQ-PCR
KW - IgH gene rearrangement
N2 - Aim. Multiple myeloma (MM) is a malignant lymphoproliferative disease of terminally differentiated B lymphocytes, characterized by expansion of monoclonal plasma cells. It is the second most common hematological cancer in the world. The introduction of novel drugs is slowly turning MM into a chronic disease. The aim of treatment is hematological remission and eradication of clinical manifestation. Nevertheless, most MM patients eventually relapse. For this reason, research is focused on more accurate monitoring of remission and relapse by molecular biology techniques. One of these techniques is allele-specific PCR and quantitative real-time PCR based on specific detection of VDJ immunoglobulin heavy chain gene rearrangement of clonal cells. The hypervariable region of IgH rearrangement is used as a marker for detection of minimal residual disease (MRD) in MM as this sequence is used for allele-specific primers and probe design. This technique is a complementary tool for flow cytometry in MRD detection in MM. The aim of this study was to introduce detection of MRD by PCR in the Czech Republic. Results. We successfully introduced qualitative and quantitative detection of a tumor marker for MRD assessment of MM by PCR in our laboratory.
ER -
SEDLAŘÍKOVÁ, Lenka, Lenka BEŠŠE, Fedor KRYUKOV, Jana PELCOVA, Zdeněk ADAM, Luděk POUR, Roman HÁJEK and Sabina ŠEVČÍKOVÁ. Detection of tumor-specific marker for minimal residual disease in multiple myeloma patients. \textit{Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic}. Olomouc: Palacký University, 2015, vol.~159, No~4, p.~554-561. ISSN~1213-8118. Available from: https://dx.doi.org/10.5507/bp.2014.035.
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