| KILLER, Jiří, Soňa DUBNÁ, Ivo SEDLÁČEK a Pavel ŠVEC. Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood. International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology, 2014, roč. 64, January, s. 152-157. ISSN 1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.053033-0. |
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@article{1192601,
author = {Killer, Jiří and Dubná, Soňa and Sedláček, Ivo and Švec, Pavel},
article_number = {January},
doi = {http://dx.doi.org/10.1099/ijs.0.053033-0},
keywords = {Lactobacillus apis sp. nov.; Apis mellifera; taxonomy},
language = {eng},
issn = {1466-5026},
journal = {International Journal of Systematic and Evolutionary Microbiology},
title = {Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood},
url = {http://ijs.sgmjournals.org/content/64/Pt_1/152.abstract?sid=bc11a653-d638-446b-b762-9ec0c3cdb393},
volume = {64},
year = {2014}
}
TY - JOUR
ID - 1192601
AU - Killer, Jiří - Dubná, Soňa - Sedláček, Ivo - Švec, Pavel
PY - 2014
TI - Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood
JF - International Journal of Systematic and Evolutionary Microbiology
VL - 64
IS - January
SP - 152-157
EP - 152-157
PB - Society for General Microbiology
SN - 14665026
KW - Lactobacillus apis sp. nov.
KW - Apis mellifera
KW - taxonomy
UR - http://ijs.sgmjournals.org/content/64/Pt_1/152.abstract?sid=bc11a653-d638-446b-b762-9ec0c3cdb393
N2 - A taxonomic study was performed on Gram-stain-positive, catalase-negative and regular rod-shaped bacterial strains R4BT and R4C, isolated from the stomachs of honeybees. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strains was within the genus Lactobacillus; the highest sequence similarity to R4BT was shown by Lactobacillus acidophilus BCRC 10695T. Lower sequence similarities were found to other obligately homofermentative lactobacilli. A PCR–DGGE method could detect the sequence of the 16S rRNA gene of strain R4BT at different developmental stages of honeybees occurring in two different locations in the Czech Republic. The distinctiveness of the strains from other lactobacilli was also confirmed by analysis of sequences of other phylogenetic markers applicable to the taxonomy of the genus Lactobacillus, ribotyping and rep-PCR analysis. The DNA G+C content of strain R4BT was 41.3% mol. The major polar lipids of strain R4BT were glycolipids, lipids and phospholipids. Phenotypic and phylogenetic characteristics also confirmed the independent status of the strains at the species level. Interestingly, strain R4BT was able to inhibit growth in vitro of Paenibacillus larvae subsp. larvae (causal agent of American foulbrood in honeybees) and Melissococcus plutonius (causal agent of European foulbrood). The name Lactobacillus apis sp. nov. is proposed for this novel taxon.
ER -
KILLER, Jiří, Soňa DUBNÁ, Ivo SEDLÁČEK a Pavel ŠVEC. Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood. \textit{International Journal of Systematic and Evolutionary Microbiology}. Society for General Microbiology, 2014, roč.~64, January, s.~152-157. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.053033-0.
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