CHLEBOWICZ, Monika A., Ivana MAŠLAŇOVÁ, Lucie KUNTOVÁ, Hajo GRUNDMANN, Roman PANTŮČEK, Jiří DOŠKAŘ, Jan Maarten VAN DIJL a Girbe BUIST. The Staphylococcal Cassette Chromosome mec type V from Staphylococcus aureus ST398 is packaged into bacteriophage capsids. International Journal of Medical Microbiology. Elsevier GmbH., 2014, roč. 304, 5-6, s. 764-774. ISSN 1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2014.05.010. |
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@article{1194951, author = {Chlebowicz, Monika A. and Mašlaňová, Ivana and Kuntová, Lucie and Grundmann, Hajo and Pantůček, Roman and Doškař, Jiří and van Dijl, Jan Maarten and Buist, Girbe}, article_number = {5-6}, doi = {http://dx.doi.org/10.1016/j.ijmm.2014.05.010}, keywords = {Staphylococcus aureus; ST398; SCCmec; Transduction; Bacteriophage}, language = {eng}, issn = {1438-4221}, journal = {International Journal of Medical Microbiology}, title = {The Staphylococcal Cassette Chromosome mec type V from Staphylococcus aureus ST398 is packaged into bacteriophage capsids}, url = {http://www.sciencedirect.com/science/article/pii/S1438422114000617}, volume = {304}, year = {2014} }
TY - JOUR ID - 1194951 AU - Chlebowicz, Monika A. - Mašlaňová, Ivana - Kuntová, Lucie - Grundmann, Hajo - Pantůček, Roman - Doškař, Jiří - van Dijl, Jan Maarten - Buist, Girbe PY - 2014 TI - The Staphylococcal Cassette Chromosome mec type V from Staphylococcus aureus ST398 is packaged into bacteriophage capsids JF - International Journal of Medical Microbiology VL - 304 IS - 5-6 SP - 764-774 EP - 764-774 PB - Elsevier GmbH. SN - 14384221 KW - Staphylococcus aureus KW - ST398 KW - SCCmec KW - Transduction KW - Bacteriophage UR - http://www.sciencedirect.com/science/article/pii/S1438422114000617 L2 - http://www.sciencedirect.com/science/article/pii/S1438422114000617 N2 - The Staphylococcal Cassette Chromosome mec (SCCmec) confers methicillin resistance to Staphylococcus aureus. While SCCmec is generally regarded as a mobile genetic element, the precise mechanisms by which large SCCmec elements are exchanged between staphylococci have remained enigmatic. In the present studies, we observed that the clinical methicillin-resistant S. aureus (MRSA) isolate UMCG-M4 with the sequence type 398 contains four prophages belonging to the serological groups A, B and Fa. Previous studies have shown that certain serological group B bacteriophages of S. aureus are capable of generalized transduction. We therefore assessed the transducing capabilities of the phages from strain UMCG-M4. The results show that some of these phages can indeed transduce plasmid pT181 to the recipient S. aureus strain RN4220. Therefore, we also investigated the possible involvement of these transducing phages in the transmission of the large SCCmec type V (5C2&5) element of S. aureus UMCG-M4. While no transduction of the complete SCCmec element was observed, we were able to demonstrate that purified phage particles did contain large parts of the SCCmec element of the donor strain, including the methicillin resistance gene mecA. This shows that staphylococcal phages can encapsulate the resistance determinant mecA of a large SCCmec type V (5C2&5) element, which may lead to its transfer to other staphylococci. ER -
CHLEBOWICZ, Monika A., Ivana MAŠLAŇOVÁ, Lucie KUNTOVÁ, Hajo GRUNDMANN, Roman PANTŮČEK, Jiří DOŠKAŘ, Jan Maarten VAN DIJL a Girbe BUIST. The Staphylococcal Cassette Chromosome mec type V from Staphylococcus aureus ST398 is packaged into bacteriophage capsids. \textit{International Journal of Medical Microbiology}. Elsevier GmbH., 2014, roč.~304, 5-6, s.~764-774. ISSN~1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2014.05.010.
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