2014
Multiplex panels of polymorphic microsatellite loci for bat bug (Cimex pipistrelli) in Central Europe.
WAWROCKA, Kamila Joanna a Tomáš BARTONIČKAZákladní údaje
Originální název
Multiplex panels of polymorphic microsatellite loci for bat bug (Cimex pipistrelli) in Central Europe.
Název anglicky
Multiplex panels of polymorphic microsatellite loci for bat bug (Cimex pipistrelli) in Central Europe.
Autoři
WAWROCKA, Kamila Joanna a Tomáš BARTONIČKA
Vydání
Xth European Congress of Entomology, University of York, UK. 2014
Další údaje
Typ výsledku
Konferenční abstrakt
Utajení
není předmětem státního či obchodního tajemství
Klíčová slova česky
bat bug, microsatelites, host
Klíčová slova anglicky
bat bug, microsatelites, host
Příznaky
Mezinárodní význam
Změněno: 7. 9. 2014 13:54, Kamila Joanna Willems, M.Sc.
Anotace
Anglicky
Bat bug (Cimex pipistrelli) is an obligatory blood sucking ectoparasite that feeds mainly on bats. It’s distribution and genetic variability in Europe is still poorly described. Previous research on Cimex pipistrelli shown morphological but no genetic diversification in bat bug according to different hosts species. Because highly polymorphic genetic markers provide useful knowledge in studies of evolution of insects, to investigate this issue further using 454 pyrosequencing a set of microsatellite markers was developed. Panels composed of 2 multiplexes was created and after analysis 7 polymorphic loci was established. Microsatelites were studied on i) 206 samples from 22 different localities (16 in Czech Republic, 5 in Slovakia, 1 in Ukraine) for geographical variability study and ii) on 67 samples from three different bat genus (Pipistrellus, Nyctalus, Myotis) for study on host diversity. All loci can be analysed in a single fragment analysis and can be used as markers to the study of evolution and the ecology of structured bug populations.