Detection of Aerosolized Biological Agents Using the
Piezoelectric Immunosensor

J 2014

Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor

KOVÁŘ, David, Zdeněk FARKA a Petr SKLÁDAL

Základní údaje

Originální název

Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor

Autoři

KOVÁŘ, David (203 Česká republika, domácí), Zdeněk FARKA (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2014, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.636

Kód RIV

RIV/00216224:14740/14:00076277

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/ac501623m

UT WoS

000341229200026

Klíčová slova anglicky

bioaerosol; quartz crystal microbalance; immunosensor; E. coli K-12; bioaerosol chamber

Štítky

kontrola MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 9. 2017 11:02, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

KOVÁŘ, David, Zdeněk FARKA a Petr SKLÁDAL. Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2014, roč. 86, č. 17, s. 8680-8686. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/ac501623m.

@article{1196466,
   author = {Kovář, David and Farka, Zdeněk and Skládal, Petr},
   article_location = {Washington, D.C., USA},
   article_number = {17},
   doi = {http://dx.doi.org/10.1021/ac501623m},
   keywords = {bioaerosol; quartz crystal microbalance; immunosensor; E. coli K-12; bioaerosol chamber},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac501623m},
   volume = {86},
   year = {2014}
}

TY  - JOUR
ID  - 1196466
AU  - Kovář, David - Farka, Zdeněk - Skládal, Petr
PY  - 2014
TI  - Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor
JF  - Analytical Chemistry
VL  - 86
IS  - 17
SP  - 8680-8686
EP  - 8680-8686
PB  - American Chemical Society
SN  - 00032700
KW  - bioaerosol
KW  - quartz crystal microbalance
KW  - immunosensor
KW  - E. coli K-12
KW  - bioaerosol chamber
UR  - http://pubs.acs.org/doi/abs/10.1021/ac501623m
L2  - http://pubs.acs.org/doi/abs/10.1021/ac501623m
N2  - Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network.
ER  -

KOVÁŘ, David, Zdeněk FARKA a Petr SKLÁDAL. Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2014, roč.~86, č.~17, s.~8680-8686. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/ac501623m.

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