An Effect of Cadmium and Lead Ions on Escherichia coli with the
Cloned Gene for Metallothionein (MT-3) Revealed by
Electrochemistry

J 2014

An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry

ADAM, Vojtěch, Dagmar CHUDOBOVÁ, Kateřina TMEJOVÁ, Kristýna ČÍHALOVÁ, Soňa KŘÍŽKOVÁ et. al.

Základní údaje

Originální název

An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry

Autoři

ADAM, Vojtěch (203 Česká republika), Dagmar CHUDOBOVÁ (203 Česká republika), Kateřina TMEJOVÁ (203 Česká republika), Kristýna ČÍHALOVÁ (203 Česká republika), Soňa KŘÍŽKOVÁ (203 Česká republika), Roman GURÁŇ (203 Česká republika), Markéta KOMÍNKOVÁ (203 Česká republika), Michal ŽŮREK (203 Česká republika), Monika KREMPLOVÁ (203 Česká republika), Ana Maria JIMENEZ JIMENEZ (203 Česká republika), Marie KONEČNÁ (203 Česká republika), David HYNEK (203 Česká republika), Vladimír PEKAŘÍK (203 Česká republika, garant, domácí) a René KIZEK (203 Česká republika)

Vydání

Electrochimica Acta, Oxford, Pergamon- Elsevier Science Ltd, 2014, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.504

Kód RIV

RIV/00216224:14740/14:00076328

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.electacta.2014.06.091

UT WoS

000342528600003

Klíčová slova anglicky

Escherichia coli; Metallothionein; Voltammetry; Cloning; Metal Ions

Štítky

kontrola MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 4. 2015 10:34, Martina Prášilová

Anotace

V originále

This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 mikroM). Bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like model organisms for studying of metals influence to metallothionein expression. Metallothionein was isolated using fast protein liquid chromatography and quantified by electrochemical methods. The occurrence of metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15 (MT dimer) and 22 kDa (MT trimer). The changes in electrochemical records due to the interactions of metallothioneins (MT-3 and MT-2A) with cadmium and lead ions showed decline of Cat2 signal of MT with the increasing interaction time because of metal ions binding to cysteines. Electrochemical determination also revealed that Cd(II) remains in E. coli cells in the higher amount than Pb (II). Opposite situation was found at E. coli–MT-3 strain. The antimicrobial effect of cadmium ions was determined by IC50 and was statistically calculated as 39.2 and 95.5 mikroM for E. coli without cloned MT-3 and E. coli carrying MT-3 gene, respectively. High provided concentration IC50 in strains after lead ions application (352.5 mikroM for E. coli without cloning and 207.0 mikroM for E. coli carrying cloned MT-3 gene) indicates lower toxicity of lead ions on bacterial strains compared to the cadmium ions.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

ADAM, Vojtěch, Dagmar CHUDOBOVÁ, Kateřina TMEJOVÁ, Kristýna ČÍHALOVÁ, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Markéta KOMÍNKOVÁ, Michal ŽŮREK, Monika KREMPLOVÁ, Ana Maria JIMENEZ JIMENEZ, Marie KONEČNÁ, David HYNEK, Vladimír PEKAŘÍK a René KIZEK. An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry. Electrochimica Acta. Oxford: Pergamon- Elsevier Science Ltd, 2014, roč. 140, September, s. 11-19. ISSN 0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2014.06.091.

@article{1196637,
   author = {Adam, Vojtěch and Chudobová, Dagmar and Tmejová, Kateřina and Číhalová, Kristýna and Křížková, Soňa and Guráň, Roman and Komínková, Markéta and Žůrek, Michal and Kremplová, Monika and Jimenez Jimenez, Ana Maria and Konečná, Marie and Hynek, David and Pekařík, Vladimír and Kizek, René},
   article_location = {Oxford},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.electacta.2014.06.091},
   keywords = {Escherichia coli; Metallothionein; Voltammetry; Cloning; Metal Ions},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry},
   url = {http://www.sciencedirect.com/science/article/pii/S0013468614012730},
   volume = {140},
   year = {2014}
}

TY  - JOUR
ID  - 1196637
AU  - Adam, Vojtěch - Chudobová, Dagmar - Tmejová, Kateřina - Číhalová, Kristýna - Křížková, Soňa - Guráň, Roman - Komínková, Markéta - Žůrek, Michal - Kremplová, Monika - Jimenez Jimenez, Ana Maria - Konečná, Marie - Hynek, David - Pekařík, Vladimír - Kizek, René
PY  - 2014
TI  - An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry
JF  - Electrochimica Acta
VL  - 140
IS  - September
SP  - 11-19
EP  - 11-19
PB  - Pergamon- Elsevier Science Ltd
SN  - 00134686
KW  - Escherichia coli
KW  - Metallothionein
KW  - Voltammetry
KW  - Cloning
KW  - Metal Ions
UR  - http://www.sciencedirect.com/science/article/pii/S0013468614012730
L2  - http://www.sciencedirect.com/science/article/pii/S0013468614012730
N2  - This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 mikroM). Bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like model organisms for studying of metals influence to metallothionein expression. Metallothionein was isolated using fast protein liquid chromatography and quantified by electrochemical methods. The occurrence of metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15 (MT dimer) and 22 kDa (MT trimer). The changes in electrochemical records due to the interactions of metallothioneins (MT-3 and MT-2A) with cadmium and lead ions showed decline of Cat2 signal of MT with the increasing interaction time because of metal ions binding to cysteines. Electrochemical determination also revealed that Cd(II) remains in E. coli cells in the higher amount than Pb (II). Opposite situation was found at E. coli–MT-3 strain. The antimicrobial effect of cadmium ions was determined by IC50 and was statistically calculated as 39.2 and 95.5 mikroM for E. coli without cloned MT-3 and E. coli carrying MT-3 gene, respectively. High provided concentration IC50 in strains after lead ions application (352.5 mikroM for E. coli without cloning and 207.0 mikroM for E. coli carrying cloned MT-3 gene) indicates lower toxicity of lead ions on bacterial strains compared to the cadmium ions.
ER  -

ADAM, Vojtěch, Dagmar CHUDOBOVÁ, Kateřina TMEJOVÁ, Kristýna ČÍHALOVÁ, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Markéta KOMÍNKOVÁ, Michal ŽŮREK, Monika KREMPLOVÁ, Ana Maria JIMENEZ JIMENEZ, Marie KONEČNÁ, David HYNEK, Vladimír PEKAŘÍK a René KIZEK. An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry. \textit{Electrochimica Acta}. Oxford: Pergamon- Elsevier Science Ltd, 2014, roč.~140, September, s.~11-19. ISSN~0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2014.06.091.

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