2014
Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK a Jakub BERKAZákladní údaje
Originální název
Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
Název česky
Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
Autoři
HYRŠL, Pavel (203 Česká republika, garant, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Libor VOJTEK (203 Česká republika, domácí) a Jakub BERKA (203 Česká republika)
Vydání
Sixth European Conference of Apidology, 2014
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30102 Immunology
Stát vydavatele
Španělsko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/14:00073256
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-84-697-0855-2
Klíčová slova česky
entomopatogenní hlístovky; včela medonosná; Photorhabdus luminescens; Heterorhabditis bacteriophora
Klíčová slova anglicky
entomopathogenic nematodes; honeaybee; Photorhabdus luminescens; Heterorhabditis bacteriophora
Změněno: 27. 9. 2014 23:55, Mgr. Pavel Dobeš, Ph.D.
V originále
Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx. 5 EPNs/larva. Infection with H. bacteriophora can be visualised using GFP labelled symbiotic bacteria or by bioluminescence. Mortality of honeybee larvae was dependent on EPN dose. Higher EPN dose also resulted in higher amount of invaded parasites. Non-segmented larvae are more susceptible to the infection than segmented larvae. We hope that this standardized method can be used as a valuable tool that will help us to describe the overall status of honeybees' immunity. Our research is supported by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047) and by the program CZ.1.07/2.3.00/30.009 co-financed from European Social Fund and the state budget of the Czech Republic.
Česky
Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx. 5 EPNs/larva. Infection with H. bacteriophora can be visualised using GFP labelled symbiotic bacteria or by bioluminescence. Mortality of honeybee larvae was dependent on EPN dose. Higher EPN dose also resulted in higher amount of invaded parasites. Non-segmented larvae are more susceptible to the infection than segmented larvae. We hope that this standardized method can be used as a valuable tool that will help us to describe the overall status of honeybees' immunity. Our research is supported by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047) and by the program CZ.1.07/2.3.00/30.009 co-financed from European Social Fund and the state budget of the Czech Republic.
Návaznosti
| CZ.1.07/2.3.00/30.0009, interní kód MU (Kód CEP: EE2.3.30.0009) |
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| QJ1210047, projekt VaV |
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