Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity

HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK a Jakub BERKA. Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity. In Sixth European Conference of Apidology. 2014. ISBN 978-84-697-0855-2.

Základní údaje

• Originální název: Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
• Název česky: Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
• Autoři: HYRŠL, Pavel (203 Česká republika, garant, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Libor VOJTEK (203 Česká republika, domácí) a Jakub BERKA (203 Česká republika).
• Vydání: Sixth European Conference of Apidology, 2014.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 30102 Immunology
• Stát vydavatele: Španělsko
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/14:00073256
• Organizační jednotka: Přírodovědecká fakulta
• ISBN: 978-84-697-0855-2
• Klíčová slova česky: entomopatogenní hlístovky; včela medonosná; Photorhabdus luminescens; Heterorhabditis bacteriophora
• Klíčová slova anglicky: entomopathogenic nematodes; honeaybee; Photorhabdus luminescens; Heterorhabditis bacteriophora

Anotace

Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx. 5 EPNs/larva. Infection with H. bacteriophora can be visualised using GFP labelled symbiotic bacteria or by bioluminescence. Mortality of honeybee larvae was dependent on EPN dose. Higher EPN dose also resulted in higher amount of invaded parasites. Non-segmented larvae are more susceptible to the infection than segmented larvae. We hope that this standardized method can be used as a valuable tool that will help us to describe the overall status of honeybees' immunity. Our research is supported by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047) and by the program CZ.1.07/2.3.00/30.009 co-financed from European Social Fund and the state budget of the Czech Republic.

Anotace česky

Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx. 5 EPNs/larva. Infection with H. bacteriophora can be visualised using GFP labelled symbiotic bacteria or by bioluminescence. Mortality of honeybee larvae was dependent on EPN dose. Higher EPN dose also resulted in higher amount of invaded parasites. Non-segmented larvae are more susceptible to the infection than segmented larvae. We hope that this standardized method can be used as a valuable tool that will help us to describe the overall status of honeybees' immunity. Our research is supported by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047) and by the program CZ.1.07/2.3.00/30.009 co-financed from European Social Fund and the state budget of the Czech Republic.

Návaznosti

• CZ.1.07/2.3.00/30.0009, interní kód MU(Kód CEP: EE2.3.30.0009): Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci (Akronym: Postdoc I.)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci, 2.3 Lidské zdroje ve výzkumu a vývoji

• QJ1210047, projekt VaV: Název: Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění (Akronym: Probiotika - imunita včel)

Investor: Ministerstvo zemědělství ČR, Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění