Comparison of the effects of silver phosphate and selenium
nanoparticles on Staphylococcus aureus growth reveals potential
for selenium particles to prevent infection

J 2014

Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection

CHUDOBOVÁ, Dagmar, Kristyna CIHALOVA, Simona DOSTALOVA, Branislav RUTTKAY-NEDECKY, Miguel Angel Merlos RODRIGO et. al.

Basic information

Original name

Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection

Authors

CHUDOBOVÁ, Dagmar (203 Czech Republic), Kristyna CIHALOVA (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Branislav RUTTKAY-NEDECKY (203 Czech Republic), Miguel Angel Merlos RODRIGO (203 Czech Republic), Kateřina TMEJOVÁ (203 Czech Republic), Pavel KOPEL (203 Czech Republic), Lukas NEJDL (203 Czech Republic), Jiri KUDR (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Soňa KŘÍŽKOVÁ (203 Czech Republic), Jindřich KYNICKÝ (203 Czech Republic), René KIZEK (203 Czech Republic) and Vojtěch ADAM (203 Czech Republic, guarantor)

Edition

FEMS Microbiology Letters, Amsterdam, Elsevier Science B. V. 2014, 0378-1097

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.121

RIV identification code

RIV/00216224:14110/14:00076562

Organization unit

Faculty of Medicine

UT WoS

000331783000012

Keywords in English

BIOFILM FORMATION; IN-VITRO; ANTIBACTERIAL; MECHANISMS; GENERATION; PATHOGENS; COATINGS; EFFICACY; PROTEIN; CELLS

Abstract

V originále

Interactions of silver phosphate nanoparticles (SPNPs) and selenium nanoparticles (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular and protein level. Significant antibacterial effects of both SPNPs and SeNPs on S. aureus were observed. At a concentration of 300 uM, SPNPs caused 37.5% inhibition of bacterial growth and SeNPs totally inhibited bacterial growth. As these effects might have been performed due to the interactions of nanoparticles with DNA and proteins, the interaction of SPNPs or SeNPs with the amplified zntR gene was studied. The presence of nanoparticles decreased the melting temperatures of the nanoparticle complexes with the zntR gene by 23% for SeNPs and by 12% for SPNPs in comparison with the control value. The concentration of bacterial metallothionein was 87% lower in bacteria after application of SPNPs (6.3 ug mg-1 protein) but was increased by 29% after addition of SeNPs (63 ug mg-1 protein) compared with the S. aureus control (49 ug mg-1 protein). Significant antimicrobial effects of the nanoparticles on bacterial growth and DNA integrity provide a promising approach to reducing the risk of bacterial infections that cannot be controlled by the usual antibiotic treatments.

Citovat

CHUDOBOVÁ, Dagmar, Kristyna CIHALOVA, Simona DOSTALOVA, Branislav RUTTKAY-NEDECKY, Miguel Angel Merlos RODRIGO, Kateřina TMEJOVÁ, Pavel KOPEL, Lukas NEJDL, Jiri KUDR, Jaromír GUMULEC, Soňa KŘÍŽKOVÁ, Jindřich KYNICKÝ, René KIZEK and Vojtěch ADAM. Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection. FEMS Microbiology Letters. Amsterdam: Elsevier Science B. V., 2014, vol. 351, No 2, p. 195-201. ISSN 0378-1097.

@article{1199010,
   author = {Chudobová, Dagmar and Cihalova, Kristyna and Dostalova, Simona and RuttkayandNedecky, Branislav and Rodrigo, Miguel Angel Merlos and Tmejová, Kateřina and Kopel, Pavel and Nejdl, Lukas and Kudr, Jiri and Gumulec, Jaromír and Křížková, Soňa and Kynický, Jindřich and Kizek, René and Adam, Vojtěch},
   article_location = {Amsterdam},
   article_number = {2},
   keywords = {BIOFILM FORMATION; IN-VITRO; ANTIBACTERIAL; MECHANISMS; GENERATION; PATHOGENS; COATINGS; EFFICACY; PROTEIN; CELLS},
   language = {eng},
   issn = {0378-1097},
   journal = {FEMS Microbiology Letters},
   title = {Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection},
   volume = {351},
   year = {2014}
}

TY  - JOUR
ID  - 1199010
AU  - Chudobová, Dagmar - Cihalova, Kristyna - Dostalova, Simona - Ruttkay-Nedecky, Branislav - Rodrigo, Miguel Angel Merlos - Tmejová, Kateřina - Kopel, Pavel - Nejdl, Lukas - Kudr, Jiri - Gumulec, Jaromír - Křížková, Soňa - Kynický, Jindřich - Kizek, René - Adam, Vojtěch
PY  - 2014
TI  - Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection
JF  - FEMS Microbiology Letters
VL  - 351
IS  - 2
SP  - 195-201
EP  - 195-201
PB  - Elsevier Science B. V.
SN  - 03781097
KW  - BIOFILM FORMATION
KW  - IN-VITRO
KW  - ANTIBACTERIAL
KW  - MECHANISMS
KW  - GENERATION
KW  - PATHOGENS
KW  - COATINGS
KW  - EFFICACY
KW  - PROTEIN
KW  - CELLS
N2  - Interactions of silver phosphate nanoparticles (SPNPs) and selenium nanoparticles (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular and protein level. Significant antibacterial effects of both SPNPs and SeNPs on S. aureus were observed. At a concentration of 300 uM, SPNPs caused 37.5% inhibition of bacterial growth and SeNPs totally inhibited bacterial growth. As these effects might have been performed due to the interactions of nanoparticles with DNA and proteins, the interaction of SPNPs or SeNPs with the amplified zntR gene was studied. The presence of nanoparticles decreased the melting temperatures of the nanoparticle complexes with the zntR gene by 23% for SeNPs and by 12% for SPNPs in comparison with the control value. The concentration of bacterial metallothionein was 87% lower in bacteria after application of SPNPs (6.3 ug mg-1 protein) but was increased by 29% after addition of SeNPs (63 ug mg-1 protein) compared with the S. aureus control (49 ug mg-1 protein). Significant antimicrobial effects of the nanoparticles on bacterial growth and DNA integrity provide a promising approach to reducing the risk of bacterial infections that cannot be controlled by the usual antibiotic treatments.
ER  -

CHUDOBOVÁ, Dagmar, Kristyna CIHALOVA, Simona DOSTALOVA, Branislav RUTTKAY-NEDECKY, Miguel Angel Merlos RODRIGO, Kateřina TMEJOVÁ, Pavel KOPEL, Lukas NEJDL, Jiri KUDR, Jaromír GUMULEC, Soňa KŘÍŽKOVÁ, Jindřich KYNICKÝ, René KIZEK and Vojtěch ADAM. Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection. \textit{FEMS Microbiology Letters}. Amsterdam: Elsevier Science B. V., 2014, vol.~351, No~2, p.~195-201. ISSN~0378-1097.

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