Capacity of Antarctic lichen Xanthoria elegans to synthetize
photoprotective secondary compounds is affected by supplemetary
UV-B doses

a 2014

Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses

BALARINOVÁ, Kateřina, Jana HAZDROVÁ a Miloš BARTÁK

Základní údaje

Originální název

Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses

Název česky

Kapacita syntézy fotoochranných sekundátních metabolitů je u lišejníku Xantoria elegans ovlivněna dávlou UV-B záření

Autoři

BALARINOVÁ, Kateřina (203 Česká republika, garant, domácí), Jana HAZDROVÁ (203 Česká republika, domácí) a Miloš BARTÁK (203 Česká republika, domácí)

Vydání

2014

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Downloadable Book of Abstracts

Kód RIV

RIV/00216224:14310/14:00076586

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7394-463-6

Klíčová slova česky

UV-B absorbující látky; fotoinhibice; ostrov Jamese Rosse; fluorescence chlorofylu; spektroskopie

Klíčová slova anglicky

UV-B screens; photoinhibition; James Ross Island;chlorophyll fluorescence; spectroscopy

Změněno: 25. 9. 2014 10:38, prof. Ing. Miloš Barták, CSc.

Anotace

ORIG CZ

V originále

Lichen thalli of X. elegans were collected at James Ross Island (Antarctica). They were dried under natural field conditions and then transported to the laboratory in Brno, Czech Republic. Before experiments, the thalli were re-wetted for 48 h by demineralized water and wet cellulose sheet located beneath the thalli. During re-wetting, the thalli were exposed to a dim light (20 micromol m-2 s-1 of PAR) at 5oC. Duration of exposition to free supplemental UV-B was 7 days. Three different intensities of UV-B radiaton were used (0.9 W.m-2, 1.4 W.m-2 and 3.0 W.m-2, in the second cycle 0.9 W.m-2, 1.15 W.m-2, 1.4 W.m-2). Samples for further analysis were taken during the exposition after 5, 10, 24, 48 and 168 h. During the exposition chlorophyll fluorescence parameters were measured using an approach of Kautsky kinetics supplemented with quenching analysis. Time courses of the following parameters were measured: Fv/Fm, Yield PSII, non-photochemical quenching (NPQ, qN) during exposition: 5, 10, 24, 48, and 168 h. After the exposition, UV-B absorbing compounds, pigment contents and concentration of glutathione were measured. UV-B induced synthesis of photoprotective compounds was measured using spectral absorption curves of extracts made in ethanol extracts. Amount of UV-B absorbing compounds was then evaluated according to the analysis of spectral absorbance curves. Chlorophyll and carotenoid contents were measured by a spectrophotometre (Specord, Analytik Jena). Glutathione was analysed using a method of thiol groups labeling.

Česky

Prosím, přečtěte si abstrakt v angličtině.

Návaznosti

LM2010009, projekt VaV

Název: Projekt CzechPolar - České polární stanice: Stavba a operační náklady

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar - České polární stanice: Stavba a operační náklady

Citovat

BALARINOVÁ, Kateřina, Jana HAZDROVÁ a Miloš BARTÁK. Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses. 2014. ISBN 978-80-7394-463-6.

@proceedings{1199186,
   author = {Balarinová, Kateřina and Hazdrová, Jana and Barták, Miloš},
   keywords = {UV-B screens; photoinhibition; James Ross Island;chlorophyll fluorescence; spectroscopy},
   language = {eng},
   isbn = {978-80-7394-463-6},
   title = {Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses},
   url = {http://polar.prf.jcu.cz/conference_MATERIALS.htm},
   year = {2014}
}

TY  - CONF
ID  - 1199186
AU  - Balarinová, Kateřina - Hazdrová, Jana - Barták, Miloš
PY  - 2014
TI  - Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses
SN  - 9788073944636
KW  - UV-B screens
KW  - photoinhibition
KW  - James Ross Island;chlorophyll fluorescence
KW  - spectroscopy
UR  - http://polar.prf.jcu.cz/conference_MATERIALS.htm
L2  - http://polar.prf.jcu.cz/conference_MATERIALS.htm
N2  - Lichen thalli of X. elegans were collected at James Ross Island (Antarctica). They were dried under natural field conditions and then transported to the laboratory in Brno, Czech Republic. Before experiments, the thalli were re-wetted for 48 h by demineralized water and wet cellulose sheet located beneath the thalli. During re-wetting, the thalli were exposed to a dim light (20 micromol m-2 s-1 of PAR) at 5oC. Duration of exposition to free supplemental UV-B was 7 days. Three different intensities of UV-B radiaton were used (0.9 W.m-2, 1.4 W.m-2 and 3.0 W.m-2, in the second cycle 0.9 W.m-2, 1.15 W.m-2, 1.4 W.m-2). Samples for further analysis were taken during the exposition after 5, 10, 24, 48 and 168 h. During the exposition chlorophyll fluorescence parameters were measured using an approach of Kautsky kinetics supplemented with quenching analysis. Time courses of the following parameters were measured: Fv/Fm, Yield PSII, non-photochemical quenching (NPQ, qN) during exposition: 5, 10, 24, 48, and 168 h. After the exposition, UV-B absorbing compounds, pigment contents and concentration of glutathione were measured. UV-B induced synthesis of photoprotective compounds was measured using spectral absorption curves of extracts made in ethanol extracts. Amount of UV-B absorbing compounds was then evaluated according to the analysis of spectral absorbance curves. Chlorophyll and carotenoid contents were measured by a spectrophotometre (Specord, Analytik Jena). Glutathione was analysed using a method of thiol groups labeling.
ER  -

BALARINOVÁ, Kateřina, Jana HAZDROVÁ a Miloš BARTÁK. \textit{Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses}. 2014. ISBN~978-80-7394-463-6.

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