Pseudogymnoascus destructans: Evidence of Virulent Skin
Invasion for Bats Under Natural Conditions, Europe

J 2015

Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe

BANDOUCHOVA, H., Tomáš BARTONIČKA, H. BERKOVA, J. BRICHTA, J. CERNY et. al.

Základní údaje

Originální název

Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe

Autoři

BANDOUCHOVA, H. (203 Česká republika), Tomáš BARTONIČKA (203 Česká republika, garant, domácí), H. BERKOVA (203 Česká republika), J. BRICHTA (203 Česká republika), J. CERNY (203 Česká republika), V. KOVACOVA (203 Česká republika), M. KOLARIK (203 Česká republika), B. KÖLLNER (203 Česká republika), P. KULICH (203 Česká republika), Natália MARTÍNKOVÁ (203 Česká republika, domácí), Zdeněk ŘEHÁK (203 Česká republika, domácí), G. G. TURNER (840 Spojené státy), Jan ZUKAL (203 Česká republika, domácí) a J. PIKULA (203 Česká republika)

Vydání

Transboundary and Emerging Diseases, Hoboken, Wiley-Blackwell, 2015, 1865-1674

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.714

Kód RIV

RIV/00216224:14310/15:00080598

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1111/tbed.12282

UT WoS

000347332000001

Klíčová slova anglicky

white-nose syndrome; chiroptera; transmission electron microscopy; ultraviolet light diagnostics; morbidity; mortality

Štítky

AKR, EL OK, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2018 10:51, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

While Pseudogymnoascus destructans has been responsible for mass bat mortalities from white-nose syndrome (WNS) in North America, its virulence in Europe has been questioned. To shed the light on the issue of host–pathogen interaction between European bats and P. destructans, we examined seventeen bats emerging from the fungus-positive underground hibernacula in the Czech Republic during early spring 2013. Dual wing-membrane biopsies were taken from Barbastella barbastellus (1), Myotis daubentonii (1), Myotis emarginatus (1), Myotis myotis (11), Myotis nattereri (1) and Plecotus auritus (2) for standard histopathology and transmission electron microscopy. Non-lethal collection of suspected WNS lesions was guided by trans-illumination of the wing membranes with ultraviolet light. All bats selected for the present study were PCR-positive for P. destructans and showed microscopic findings consistent with the histopathological criteria for WNS diagnosis. Ultramicroscopy revealed oedema of the connective tissue and derangement of the fibroblasts and elastic fibres associated with skin invasion by P. destructans. Extensive fungal infection induced a marked inflammatory infiltration by neutrophils at the interface between the damaged part of the wing membrane replaced by the fungus and membrane tissue not yet invaded by the pathogen. There was no sign of keratinolytic activity in the stratum corneum. Here, we show that lesions pathognomonic for WNS are common in European bats and may also include overwhelming full-thickness fungal growth through the wing membrane equal in severity to reports from North America. Inter-continental differences in the outcome of WNS in bats in terms of morbidity/mortality may therefore not be due to differences in the pathogen itself.

Návaznosti

GAP506/12/1064, projekt VaV

Název: Adaptace netopýrů na plísňové onemocnění geomykózu

Investor: Grantová agentura ČR, Adaptace netopýrů na plísňové onemocnění geomykózou

Citovat

BANDOUCHOVA, H., Tomáš BARTONIČKA, H. BERKOVA, J. BRICHTA, J. CERNY, V. KOVACOVA, M. KOLARIK, B. KÖLLNER, P. KULICH, Natália MARTÍNKOVÁ, Zdeněk ŘEHÁK, G. G. TURNER, Jan ZUKAL a J. PIKULA. Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe. Transboundary and Emerging Diseases. Hoboken: Wiley-Blackwell, 2015, roč. 62, č. 1, s. 1-5. ISSN 1865-1674. Dostupné z: https://dx.doi.org/10.1111/tbed.12282.

@article{1200036,
   author = {Bandouchova, H. and Bartonička, Tomáš and Berkova, H. and Brichta, J. and Cerny, J. and Kovacova, V. and Kolarik, M. and Köllner, B. and Kulich, P. and Martínková, Natália and Řehák, Zdeněk and Turner, G. G. and Zukal, Jan and Pikula, J.},
   article_location = {Hoboken},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/tbed.12282},
   keywords = {white-nose syndrome; chiroptera; transmission electron microscopy; ultraviolet light diagnostics; morbidity; mortality},
   language = {eng},
   issn = {1865-1674},
   journal = {Transboundary and Emerging Diseases},
   title = {Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe},
   volume = {62},
   year = {2015}
}

TY  - JOUR
ID  - 1200036
AU  - Bandouchova, H. - Bartonička, Tomáš - Berkova, H. - Brichta, J. - Cerny, J. - Kovacova, V. - Kolarik, M. - Köllner, B. - Kulich, P. - Martínková, Natália - Řehák, Zdeněk - Turner, G. G. - Zukal, Jan - Pikula, J.
PY  - 2015
TI  - Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe
JF  - Transboundary and Emerging Diseases
VL  - 62
IS  - 1
SP  - 1-5
EP  - 1-5
PB  - Wiley-Blackwell
SN  - 18651674
KW  - white-nose syndrome
KW  - chiroptera
KW  - transmission electron microscopy
KW  - ultraviolet light diagnostics
KW  - morbidity
KW  - mortality
N2  - While Pseudogymnoascus destructans has been responsible for mass bat mortalities from white-nose syndrome (WNS) in North America, its virulence in Europe has been questioned. To shed the light on the issue of host–pathogen interaction between European bats and P. destructans, we examined seventeen bats emerging from the fungus-positive underground hibernacula in the Czech Republic during early spring 2013. Dual wing-membrane biopsies were taken from Barbastella barbastellus (1), Myotis daubentonii (1), Myotis emarginatus (1), Myotis myotis (11), Myotis nattereri (1) and Plecotus auritus (2) for standard histopathology and transmission electron microscopy. Non-lethal collection of suspected WNS lesions was guided by trans-illumination of the wing membranes with ultraviolet light. All bats selected for the present study were PCR-positive for P. destructans and showed microscopic findings consistent with the histopathological criteria for WNS diagnosis. Ultramicroscopy revealed oedema of the connective tissue and derangement of the fibroblasts and elastic fibres associated with skin invasion by P. destructans. Extensive fungal infection induced a marked inflammatory infiltration by neutrophils at the interface between the damaged part of the wing membrane replaced by the fungus and membrane tissue not yet invaded by the pathogen. There was no sign of keratinolytic activity in the stratum corneum. Here, we show that lesions pathognomonic for WNS are common in European bats and may also include overwhelming full-thickness fungal growth through the wing membrane equal in severity to reports from North America. Inter-continental differences in the outcome of WNS in bats in terms of morbidity/mortality may therefore not be due to differences in the pathogen itself.
ER  -

BANDOUCHOVA, H., Tomáš BARTONIČKA, H. BERKOVA, J. BRICHTA, J. CERNY, V. KOVACOVA, M. KOLARIK, B. KÖLLNER, P. KULICH, Natália MARTÍNKOVÁ, Zdeněk ŘEHÁK, G. G. TURNER, Jan ZUKAL a J. PIKULA. Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe. \textit{Transboundary and Emerging Diseases}. Hoboken: Wiley-Blackwell, 2015, roč.~62, č.~1, s.~1-5. ISSN~1865-1674. Dostupné z: https://dx.doi.org/10.1111/tbed.12282.

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