Detailed Information on Publication Record
2014
Using environmental scanning electron microscopy (ESEM) as a non-invasive method to studying fixed parasites
MAŠOVÁ, Šárka, Vilém NEDĚLA and Eva TIHLAŘÍKOVÁBasic information
Original name
Using environmental scanning electron microscopy (ESEM) as a non-invasive method to studying fixed parasites
Authors
MAŠOVÁ, Šárka (203 Czech Republic, guarantor, belonging to the institution), Vilém NEDĚLA (203 Czech Republic) and Eva TIHLAŘÍKOVÁ (203 Czech Republic)
Edition
18th International Microscopy Congress. 2014
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/14:00073959
Organization unit
Faculty of Science
ISBN
978-80-260-6720-7
Keywords in English
ESEM; microscopy; parasites; fishes parasites
Tags
International impact
Změněno: 3/3/2015 15:22, RNDr. Šárka Mašová, Ph.D.
Abstract
V originále
ESEM observation on several groups of already fixed parasites was made. Observations were made with the experimental environmental SEM AQUASEM-II redesigned in the Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic by Ionization and YAG-BSE detectors. The samples were cooled down to 2°C and observed in an high pressure water vapour environment of 650–700 Pa. Samples were placed on a Peltier cooled specimen holder to a drop of water. Consequently the water was slowly evaporated from the sample. In this study, we showed that ESEM allows the examination of specimens at high pressure conditions without any previous treatment in fully hydrated state and can be used effectively in taxonomical studies of the parasites, where valuable unique specimens sometimes exist.
Links
| GBP505/12/G112, research and development project |
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