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@article{1202739, author = {Matalová, Eva and Zdražilová Dubská, Lenka and Fleischmannová, Jana and Chlastakova, I and Janečková, Eva and Tucker, A S}, article_location = {Amsterodam}, article_number = {8}, doi = {http://dx.doi.org/10.1016/j.archoralbio.2010.05.007}, issn = {0003-9969}, journal = {Archives of Oral Biology}, title = {Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples}, volume = {55}, year = {2010} }
TY - JOUR ID - 1202739 AU - Matalová, Eva - Zdražilová Dubská, Lenka - Fleischmannová, Jana - Chlastakova, I - Janečková, Eva - Tucker, A S PY - 2010 TI - Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples JF - Archives of Oral Biology VL - 55 IS - 8 SP - 570-575 EP - 570-575 PB - Elsevier SN - 00039969 N2 - Laser capture microdissection (LCM) uniquely allows the selection of specific cell populations from histological sections These selected cells are then catapulted into a test tube without any contamination from surrounding tissues During the last ten years, many significant results have been achieved, particularly at the level of DNA and RNA where amplification techniques are available However, where amplification procedures are difficult, the benefits of LCM diminish To overcome such difficulties, a novel approach, combining laser capture microdissection and flow cytometry, has been tested here for detection of apoptosis and proliferation in tissue bound cell populations without any amplification steps The mouse cap stage molar tooth germ was used as a model At the centre of the inner enamel epithelium, the primary enamel knot is a clearly defined apoptotic population with minimal proliferation, flanked by the highly proliferative cervical loops on each side Thus within the tooth germ epithelium at this stage, two distinct populations of cells are found side by side These populations were selected by laser capture microdissection and then analysed by flow cytometry for apoptosis and proliferation Flow cytometric results correlated well with immunohistochemical findings, demonstrating the success and sensitivity of this combined procedure (C) 2010 Elsevier Ltd All rights reserved ER -
MATALOVÁ, Eva, Lenka ZDRAŽILOVÁ DUBSKÁ, Jana FLEISCHMANNOVÁ, I CHLASTAKOVA, Eva JANEČKOVÁ a A S TUCKER. Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples. \textit{Archives of Oral Biology}. Amsterodam: Elsevier, 2010, roč.~55, č.~8, s.~570-575. ISSN~0003-9969. Dostupné z: https://dx.doi.org/10.1016/j.archoralbio.2010.05.007.
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