| TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. Folia biologica. Praha: Univerzita Karlova, 2014, roč. 60, č. 1, s. 90-94. ISSN 0015-5500. |
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@article{1202839,
author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
article_location = {Praha},
article_number = {1},
keywords = {human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells},
language = {eng},
issn = {0015-5500},
journal = {Folia biologica},
title = {Haematopoietic developmental potential of human pluripotent stem cell lines},
volume = {60},
year = {2014}
}
TY - JOUR
ID - 1202839
AU - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY - 2014
TI - Haematopoietic developmental potential of human pluripotent stem cell lines
JF - Folia biologica
VL - 60
IS - 1
SP - 90-94
EP - 90-94
PB - Univerzita Karlova
SN - 00155500
KW - human pluripotent stem cells
KW - hematopoietic development
KW - embryoid bodies
KW - CD34+ cells
N2 - The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines.
ER -
TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. \textit{Folia biologica}. Praha: Univerzita Karlova, 2014, roč.~60, č.~1, s.~90-94. ISSN~0015-5500.
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