Piezoelectric Immunosensor for Detection of Aerosolized
Microorganisms

a 2014

Piezoelectric Immunosensor for Detection of Aerosolized Microorganisms

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL

Základní údaje

Originální název

Piezoelectric Immunosensor for Detection of Aerosolized Microorganisms

Autoři

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL

Vydání

CEITEC Annual Conference 2014: Frontiers in Material and Life Sciences, 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-210-7159-9

Klíčová slova anglicky

bioaerosol; E. coli; immunosensor; QCM

Příznaky

Mezinárodní význam

Změněno: 7. 11. 2014 08:49, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Detection of microorganisms has a crucial role in many fields. Even though the traditional microbiological methods are robust and provide low limits of detection, the analysis is usually time consuming. In some cases (e.g. monitoring of airborne pathogens), the fast detection is essential. Rapid and specific analysis can be provided by biosensors. In this work, the piezoelectric immunosensor for detection of aerosolized microorganisms was developed. Nonpathogenic E. coli K-12 was chosen as a biological warfare agents’ surrogate. The biosensing layer was prepared by immobilization of specific antibodies using bifunctional crosslinker Sulfo-SMCC. To allow safe work with aerosolized microorganisms, bioaerosol chamber was constructed. The dissemination was done using a piezoelectric aerosol generator and the samples were collected by a wetted-wall cyclone on-line coupled with the piezoelectric immunosensor. The concentration 1.45×10^4 CFU·L-1 of E. coli K-12 in air was successfully detected in 16 min. The whole measurement cycle including sensor surface regeneration and filling the chamber with clean air was completed in 40 min. Reference measurements were done using commercial particle counter and by cultivation method. The specificity was also tested on real air samples. The achieved results indicate that the developed sensor can be used for screening of microorganisms in the air.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL. Piezoelectric Immunosensor for Detection of Aerosolized Microorganisms. In CEITEC Annual Conference 2014: Frontiers in Material and Life Sciences. 2014. ISBN 978-80-210-7159-9.

@proceedings{1203470,
   author = {Farka, Zdeněk and Kovář, David and Skládal, Petr},
   booktitle = {CEITEC Annual Conference 2014: Frontiers in Material and Life Sciences},
   keywords = {bioaerosol; E. coli; immunosensor; QCM},
   language = {eng},
   isbn = {978-80-210-7159-9},
   title = {Piezoelectric Immunosensor for Detection of Aerosolized Microorganisms},
   url = {http://www.ceitec.eu/ac2014-book-of-abstract/f32414},
   year = {2014}
}

TY  - CONF
ID  - 1203470
AU  - Farka, Zdeněk - Kovář, David - Skládal, Petr
PY  - 2014
TI  - Piezoelectric Immunosensor for Detection of Aerosolized Microorganisms
SN  - 9788021071599
KW  - bioaerosol
KW  - E. coli
KW  - immunosensor
KW  - QCM
UR  - http://www.ceitec.eu/ac2014-book-of-abstract/f32414
N2  - Detection of microorganisms has a crucial role in many fields. Even though the traditional microbiological methods are robust and provide low limits of detection, the analysis is usually time consuming. In some cases (e.g. monitoring of airborne pathogens), the fast detection is essential. Rapid and specific analysis can be provided by biosensors. In this work, the piezoelectric immunosensor for detection of aerosolized microorganisms was developed. Nonpathogenic E. coli K-12 was chosen as a biological warfare agents’ surrogate. The biosensing layer was prepared by immobilization of specific antibodies using bifunctional crosslinker Sulfo-SMCC. To allow safe work with aerosolized microorganisms, bioaerosol chamber was constructed. The dissemination was done using a piezoelectric aerosol generator and the samples were collected by a wetted-wall cyclone on-line coupled with the piezoelectric immunosensor. The concentration 1.45×10^4 CFU·L-1 of E. coli K-12 in air was successfully detected in 16 min. The whole measurement cycle including sensor surface regeneration and filling the chamber with clean air was completed in 40 min. Reference measurements were done using commercial particle counter and by cultivation method. The specificity was also tested on real air samples. The achieved results indicate that the developed sensor can be used for screening of microorganisms in the air.
ER  -

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL. Piezoelectric Immunosensor for Detection of Aerosolized Microorganisms. In \textit{CEITEC Annual Conference 2014: Frontiers in Material and Life Sciences}. 2014. ISBN~978-80-210-7159-9.

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