CHUDOBOVA, Dagmar, Kristyna CIHALOVA, Roman GURÁŇ, Simona DOSTALOVA, Kristyna SMERKOVA, Radek VESELÝ, Jaromír GUMULEC, Zbynek HEGER, Michal MASAŘÍK, Vojtěch ADAM and René KIZEK. IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING. In František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník. CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis. 1st ed. Brno: Institute of Analytical Chemistry AS CR, 2014, p. 178-181. ISBN 978-80-904959-2-0.
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Basic information
Original name IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING
Authors CHUDOBOVA, Dagmar (203 Czech Republic), Kristyna CIHALOVA (203 Czech Republic), Roman GURÁŇ (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Kristyna SMERKOVA (203 Czech Republic), Radek VESELÝ (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Zbynek HEGER (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Vojtěch ADAM (203 Czech Republic) and René KIZEK (203 Czech Republic).
Edition 1. vyd. Brno, CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis, p. 178-181, 4 pp. 2014.
Publisher Institute of Analytical Chemistry AS CR
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
WWW URL
RIV identification code RIV/00216224:14110/14:00076945
Organization unit Faculty of Medicine
ISBN 978-80-904959-2-0
Keywords in English HARD-TO-HEAL WOUNDS; MALDI-TOF MS; SEQUENCING
Tags EL OK
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 10/4/2015 16:15.
Abstract
This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was < 2.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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