A Versatile Scaffold Contributes to Damage Survival via
Sumoylation and Nuclease Interactions

J 2014

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

SARANGI, Prabha, Veronika ALTMANNOVÁ, Cory HOLLAND, Zdenka BARTOŠOVÁ, Fanfan HAO et. al.

Základní údaje

Originální název

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

Autoři

SARANGI, Prabha (840 Spojené státy), Veronika ALTMANNOVÁ (203 Česká republika, domácí), Cory HOLLAND (840 Spojené státy), Zdenka BARTOŠOVÁ (703 Slovensko, domácí), Fanfan HAO (840 Spojené státy), Dorothea ANRATHER (40 Rakousko), Gustav AMMERER (40 Rakousko), Sang Eun LEE (840 Spojené státy), Lumír KREJČÍ (203 Česká republika, garant, domácí) a Xiaolan ZHAO (840 Spojené státy)

Vydání

Cell Reports, CAMBRIDGE, Spojené státy americké, Cell Press, 2014, 2211-1247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 8.358

Kód RIV

RIV/00216224:14110/14:00074018

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.celrep.2014.08.054

UT WoS

000344468100014

Klíčová slova anglicky

STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 1. 2015 00:06, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.

Návaznosti

EE2.3.20.0011, projekt VaV

Název: Centrum výzkumu pluripotentních buněk a nestability genomu

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

Citovat

SARANGI, Prabha, Veronika ALTMANNOVÁ, Cory HOLLAND, Zdenka BARTOŠOVÁ, Fanfan HAO, Dorothea ANRATHER, Gustav AMMERER, Sang Eun LEE, Lumír KREJČÍ a Xiaolan ZHAO. A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions. Cell Reports. CAMBRIDGE, Spojené státy americké: Cell Press, 2014, roč. 9, č. 1, s. 143-152. ISSN 2211-1247. Dostupné z: https://dx.doi.org/10.1016/j.celrep.2014.08.054.

@article{1204451,
   author = {Sarangi, Prabha and Altmannová, Veronika and Holland, Cory and Bartošová, Zdenka and Hao, Fanfan and Anrather, Dorothea and Ammerer, Gustav and Lee, Sang Eun and Krejčí, Lumír and Zhao, Xiaolan},
   article_location = {CAMBRIDGE, Spojené státy americké},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.celrep.2014.08.054},
   keywords = {STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE},
   language = {eng},
   issn = {2211-1247},
   journal = {Cell Reports},
   title = {A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1204451
AU  - Sarangi, Prabha - Altmannová, Veronika - Holland, Cory - Bartošová, Zdenka - Hao, Fanfan - Anrather, Dorothea - Ammerer, Gustav - Lee, Sang Eun - Krejčí, Lumír - Zhao, Xiaolan
PY  - 2014
TI  - A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions
JF  - Cell Reports
VL  - 9
IS  - 1
SP  - 143-152
EP  - 143-152
PB  - Cell Press
SN  - 22111247
KW  - STRAND BREAK REPAIR
KW  - YEAST SCHIZOSACCHAROMYCES-POMBE
KW  - HOLLIDAY JUNCTION RESOLVASE
KW  - DNA-REPAIR
KW  - SACCHAROMYCES-CEREVISIAE
KW  - PROTEIN INTERACTIONS
KW  - RAD1-RAD10 NUCLEASE
KW  - ABASIC SITES
KW  - RECOMBINATION
KW  - ENDONUCLEASE
N2  - DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
ER  -

SARANGI, Prabha, Veronika ALTMANNOVÁ, Cory HOLLAND, Zdenka BARTOŠOVÁ, Fanfan HAO, Dorothea ANRATHER, Gustav AMMERER, Sang Eun LEE, Lumír KREJČÍ a Xiaolan ZHAO. A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions. \textit{Cell Reports}. CAMBRIDGE, Spojené státy americké: Cell Press, 2014, roč.~9, č.~1, s.~143-152. ISSN~2211-1247. Dostupné z: https://dx.doi.org/10.1016/j.celrep.2014.08.054.

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