Differential expression of anterior gradient protein 3 in
intrahepatic cholangiocarcinoma and hepatocellular carcinoma.

J 2014

Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma.

BRYCHTOVÁ, Veronika, Víta ŽAMPACHOVÁ, Roman HRSTKA, Pavel FABIAN, Jiri NOVAK et. al.

Základní údaje

Originální název

Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma.

Autoři

BRYCHTOVÁ, Veronika (203 Česká republika), Víta ŽAMPACHOVÁ (203 Česká republika, domácí), Roman HRSTKA (203 Česká republika), Pavel FABIAN (203 Česká republika), Jiri NOVAK (203 Česká republika), Markéta HERMANOVÁ (203 Česká republika, garant, domácí) a Bořivoj VOJTĚŠEK (203 Česká republika)

Vydání

Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma, San Diego, Academic Press INC Elsevier Science, 2014, 0014-4800

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.706

Kód RIV

RIV/00216224:14110/14:00077103

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.yexmp.2014.04.002

UT WoS

000337212600016

Klíčová slova anglicky

AGR3; GPC-3; Intrahepatic cholangiocarcinoma; Hepatocellular carcinoma; Mucopolysaccharides; Immunohistochemistry

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 2. 2015 12:33, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer next to hepatocellular carcinoma (HCC). Despite the significant difference of the therapeutic strategy for both diseases, their histological appearance may be very similar. Thus the correct diagnosis is crucial for treatment choice but is often difficult to achieve. The aim of our study was to evaluate anterior gradient 3 (AGR3) as a new diagnostic marker helping to distinguish between ICC and HCC. AGR3 is a putative transmembrane protein implicated in breast, prostate and ovary tumorigenesis and belongs to the family of protein disulfide isomerases. Since there is little information on how AGR3 is expressed in normal and diseased tissues and what its exact function is, we analyzed its expression pattern in normal liver and tumor tissue of ICC and HCC. The immunohistochemical analysis in normal tissue revealed specific AGR3 expression in intrahepatic bile duct cholangiocytes which was not present in liver hepatocytes. Consequently we analyzed AGR3 expression in 74 representative samples of puncture biopsies, tissue excisions and resection specimens from which 48 samples were diagnosed as HCC and 26 as ICC. Our results showed AGR3 expression negative and weakly positive respectively in hepatocellular carcinomas compared to stronger AGR3 positivity in cholangiocellular carcinomas. AGR3 expression statistically significantly correlated to acid mucopolysaccharide expression and negatively correlated to glypican-3 expression. We conclude that according to receiver operating characteristics (ROC) analysis AGR3 expression is relatively specific for ICC and is potentially linked to mucosecretion, which may indicate potential implication in treatment resistance.

Citovat

BRYCHTOVÁ, Veronika, Víta ŽAMPACHOVÁ, Roman HRSTKA, Pavel FABIAN, Jiri NOVAK, Markéta HERMANOVÁ a Bořivoj VOJTĚŠEK. Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma. Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma. San Diego: Academic Press INC Elsevier Science, 2014, roč. 96, č. 3, s. 375-381. ISSN 0014-4800. Dostupné z: https://dx.doi.org/10.1016/j.yexmp.2014.04.002.

@article{1204857,
   author = {Brychtová, Veronika and Žampachová, Víta and Hrstka, Roman and Fabian, Pavel and Novak, Jiri and Hermanová, Markéta and Vojtěšek, Bořivoj},
   article_location = {San Diego},
   article_number = {3},
   doi = {http://dx.doi.org/10.1016/j.yexmp.2014.04.002},
   keywords = {AGR3; GPC-3; Intrahepatic cholangiocarcinoma; Hepatocellular carcinoma; Mucopolysaccharides; Immunohistochemistry},
   language = {eng},
   issn = {0014-4800},
   journal = {Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma},
   title = {Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma.},
   volume = {96},
   year = {2014}
}

TY  - JOUR
ID  - 1204857
AU  - Brychtová, Veronika - Žampachová, Víta - Hrstka, Roman - Fabian, Pavel - Novak, Jiri - Hermanová, Markéta - Vojtěšek, Bořivoj
PY  - 2014
TI  - Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma.
JF  - Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma
VL  - 96
IS  - 3
SP  - 375-381
EP  - 375-381
PB  - Academic Press INC Elsevier Science
SN  - 00144800
KW  - AGR3
KW  - GPC-3
KW  - Intrahepatic cholangiocarcinoma
KW  - Hepatocellular carcinoma
KW  - Mucopolysaccharides
KW  - Immunohistochemistry
N2  - Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer next to hepatocellular carcinoma (HCC). Despite the significant difference of the therapeutic strategy for both diseases, their histological appearance may be very similar. Thus the correct diagnosis is crucial for treatment choice but is often difficult to achieve. The aim of our study was to evaluate anterior gradient 3 (AGR3) as a new diagnostic marker helping to distinguish between ICC and HCC. AGR3 is a putative transmembrane protein implicated in breast, prostate and ovary tumorigenesis and belongs to the family of protein disulfide isomerases. Since there is little information on how AGR3 is expressed in normal and diseased tissues and what its exact function is, we analyzed its expression pattern in normal liver and tumor tissue of ICC and HCC. The immunohistochemical analysis in normal tissue revealed specific AGR3 expression in intrahepatic bile duct cholangiocytes which was not present in liver hepatocytes. Consequently we analyzed AGR3 expression in 74 representative samples of puncture biopsies, tissue excisions and resection specimens from which 48 samples were diagnosed as HCC and 26 as ICC. Our results showed AGR3 expression negative and weakly positive respectively in hepatocellular carcinomas compared to stronger AGR3 positivity in cholangiocellular carcinomas. AGR3 expression statistically significantly correlated to acid mucopolysaccharide expression and negatively correlated to glypican-3 expression. We conclude that according to receiver operating characteristics (ROC) analysis AGR3 expression is relatively specific for ICC and is potentially linked to mucosecretion, which may indicate potential implication in treatment resistance.
ER  -

BRYCHTOVÁ, Veronika, Víta ŽAMPACHOVÁ, Roman HRSTKA, Pavel FABIAN, Jiri NOVAK, Markéta HERMANOVÁ a Bořivoj VOJTĚŠEK. Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma. \textit{Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma}. San Diego: Academic Press INC Elsevier Science, 2014, roč.~96, č.~3, s.~375-381. ISSN~0014-4800. Dostupné z: https://dx.doi.org/10.1016/j.yexmp.2014.04.002.

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