Generation of Human Induced Pluripotent Stem Cells Using Genome
Integrating or Non-integrating Methods

J 2014

Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Stanislava PAĎOUROVÁ a Irena KRONTORÁD KOUTNÁ

Základní údaje

Originální název

Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods

Autoři

ŠIMARA, Pavel (203 Česká republika, domácí), Lenka TESAŘOVÁ (203 Česká republika, domácí), Stanislava PAĎOUROVÁ (703 Slovensko, domácí) a Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant, domácí)

Vydání

Folia Biologica, Praha, Univerzita Karlova, 2014, 0015-5500

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.000

Kód RIV

RIV/00216224:14330/14:00074034

Organizační jednotka

Fakulta informatiky

UT WoS

000343275800014

Klíčová slova česky

hiPSCs; lentivirus; Sendai virus; episomalní reprogramace

Klíčová slova anglicky

hiPSCs; lentivirus; Sendai virus; episomal reprogramming

Štítky

cbia-web

Změněno: 2. 3. 2018 10:06, Mgr. Pavel Šimara, Ph.D.

Anotace

V originále

Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfil this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during seven years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSC clones are highly preferable in terms of future clinical application. In this commentary article, we summarize the reprogramming experiments performed in our laboratories. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human neonatal fibroblasts and CD34+ blood progenitors were used as cell sources and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Stanislava PAĎOUROVÁ a Irena KRONTORÁD KOUTNÁ. Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods. Folia Biologica. Praha: Univerzita Karlova, 2014, roč. 60, S1, s. 85-89. ISSN 0015-5500.

@article{1204941,
   author = {Šimara, Pavel and Tesařová, Lenka and Paďourová, Stanislava and Krontorád Koutná, Irena},
   article_location = {Praha},
   article_number = {S1},
   keywords = {hiPSCs; lentivirus; Sendai virus; episomal reprogramming},
   language = {eng},
   issn = {0015-5500},
   journal = {Folia Biologica},
   title = {Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods},
   volume = {60},
   year = {2014}
}

TY  - JOUR
ID  - 1204941
AU  - Šimara, Pavel - Tesařová, Lenka - Paďourová, Stanislava - Krontorád Koutná, Irena
PY  - 2014
TI  - Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods
JF  - Folia Biologica
VL  - 60
IS  - S1
SP  - 85-89
EP  - 85-89
PB  - Univerzita Karlova
SN  - 00155500
KW  - hiPSCs
KW  - lentivirus
KW  - Sendai virus
KW  - episomal reprogramming
N2  - Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfil this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during seven years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSC clones are highly preferable in terms of future clinical application. In this commentary article, we summarize the reprogramming experiments performed in our laboratories. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human neonatal fibroblasts and CD34+ blood progenitors were used as cell sources and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions.
ER  -

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Stanislava PAĎOUROVÁ a Irena KRONTORÁD KOUTNÁ. Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods. \textit{Folia Biologica}. Praha: Univerzita Karlova, 2014, roč.~60, S1, s.~85-89. ISSN~0015-5500.

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