HP1B-dependent recruitment of UBF1 to irradiated chromatin
occurs simultaneously with CPDs

J 2014

HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

STIXOVÁ, Lenka, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Tereza HRUŠKOVÁ et. al.

Základní údaje

Originální název

HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Autoři

STIXOVÁ, Lenka (203 Česká republika), Petra SEHNALOVÁ (203 Česká republika), Soňa LEGARTOVÁ (703 Slovensko), Jana SUCHÁNKOVÁ (203 Česká republika), Tereza HRUŠKOVÁ (203 Česká republika), Stanislav KOZUBEK (203 Česká republika), Dmitry SOROKIN (643 Rusko, garant, domácí), Pavel MATULA (203 Česká republika, domácí), Ivan RAŠKA (203 Česká republika), Aleš KOVAŘÍK (203 Česká republika), Jaroslav FULNEČEK (203 Česká republika) a Eva BÁRTOVÁ (203 Česká republika)

Vydání

Epigenetics & Chromatin, BioMed Central, 2014, 1756-8935

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.333

Kód RIV

RIV/00216224:14330/14:00074061

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1186/1756-8935-7-39

UT WoS

000347931500001

Klíčová slova anglicky

DNA-damage response; DNA repair; Irradiation; Live-cell studies; Nucleolus; UBF1

Štítky

CBIA, cbia-web

Změněno: 28. 4. 2015 13:35, doc. RNDr. Pavel Matula, Ph.D.

Anotace

V originále

Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. Transcription factor UBF1 also interacted directly with DNA inside the nucleolus but no interaction of UBF1 and DNA was confirmed outside the nucleolus, where UBF1 recruitment to DNA lesions appeared simultaneously with cyclobutane pyrimidine dimers; this occurrence was cell-cycle-independent. Conclusions. We propose that the simultaneous presence and interaction of UBF1 and HP1betta at DNA lesions is activated by the presence of cyclobutane pyrimidine dimers and mediated by the chromo shadow domain of HP1betta. This might have functional significance for nucleotide excision repair.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

STIXOVÁ, Lenka, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Tereza HRUŠKOVÁ, Stanislav KOZUBEK, Dmitry SOROKIN, Pavel MATULA, Ivan RAŠKA, Aleš KOVAŘÍK, Jaroslav FULNEČEK a Eva BÁRTOVÁ. HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs. Epigenetics & Chromatin. BioMed Central, 2014, roč. 7, č. 39, s. 1-17. ISSN 1756-8935. Dostupné z: https://dx.doi.org/10.1186/1756-8935-7-39.

@article{1205176,
   author = {Stixová, Lenka and Sehnalová, Petra and Legartová, Soňa and Suchánková, Jana and Hrušková, Tereza and Kozubek, Stanislav and Sorokin, Dmitry and Matula, Pavel and Raška, Ivan and Kovařík, Aleš and Fulneček, Jaroslav and Bártová, Eva},
   article_number = {39},
   doi = {http://dx.doi.org/10.1186/1756-8935-7-39},
   keywords = {DNA-damage response; DNA repair; Irradiation; Live-cell studies; Nucleolus; UBF1},
   language = {eng},
   issn = {1756-8935},
   journal = {Epigenetics & Chromatin},
   title = {HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs},
   volume = {7},
   year = {2014}
}

TY  - JOUR
ID  - 1205176
AU  - Stixová, Lenka - Sehnalová, Petra - Legartová, Soňa - Suchánková, Jana - Hrušková, Tereza - Kozubek, Stanislav - Sorokin, Dmitry - Matula, Pavel - Raška, Ivan - Kovařík, Aleš - Fulneček, Jaroslav - Bártová, Eva
PY  - 2014
TI  - HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
JF  - Epigenetics & Chromatin
VL  - 7
IS  - 39
SP  - 1-17
EP  - 1-17
PB  - BioMed Central
SN  - 17568935
KW  - DNA-damage response
KW  - DNA repair
KW  - Irradiation
KW  - Live-cell studies
KW  - Nucleolus
KW  - UBF1
N2  - Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. Transcription factor UBF1 also interacted directly with DNA inside the nucleolus but no interaction of UBF1 and DNA was confirmed outside the nucleolus, where UBF1 recruitment to DNA lesions appeared simultaneously with cyclobutane pyrimidine dimers; this occurrence was cell-cycle-independent. Conclusions. We propose that the simultaneous presence and interaction of UBF1 and HP1betta at DNA lesions is activated by the presence of cyclobutane pyrimidine dimers and mediated by the chromo shadow domain of HP1betta. This might have functional significance for nucleotide excision repair.
ER  -

STIXOVÁ, Lenka, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Tereza HRUŠKOVÁ, Stanislav KOZUBEK, Dmitry SOROKIN, Pavel MATULA, Ivan RAŠKA, Aleš KOVAŘÍK, Jaroslav FULNEČEK a Eva BÁRTOVÁ. HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs. \textit{Epigenetics \&{} Chromatin}. BioMed Central, 2014, roč.~7, č.~39, s.~1-17. ISSN~1756-8935. Dostupné z: https://dx.doi.org/10.1186/1756-8935-7-39.

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