Double opposite end injection capillary electrophoresis with
contactless conductometric detection for simultaneous
determination of chloride, sodium and potassium in cystic
fibrosis diagnosis

J 2014

Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis

KUBÁŇ, Petr; Michal GREGUŠ; Eva POKOJOVÁ; Jana SKŘIČKOVÁ; František FORET et al.

Základní údaje

Originální název

Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis

Autoři

KUBÁŇ, Petr; Michal GREGUŠ; Eva POKOJOVÁ; Jana SKŘIČKOVÁ a František FORET

Vydání

Journal of Chromatography A, AMSTERDAM, Elsevier, 2014, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.169

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/14:00074081

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Capillary electrophoresis; Contactless conductivity detection; Ions; Cystic fibrosis; Principal component analysis

Štítky

EL OK, kontrola MP, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 3. 2015 09:07, Martina Prášilová

Anotace

V originále

A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed. (C) 2014 Elsevier B.V. All rights reserved.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-21919S, projekt VaV

Název: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Grantová agentura ČR, Vývoj neinvazivních metod odberu vzorku a analýzy v point-of-care dignostice biologických materiálu

Přiložené soubory

ZVV_2014_215_1205561_Double_opposite.pdf

Požádat o autorskou verzi souboru

Citovat

KUBÁŇ, Petr; Michal GREGUŠ; Eva POKOJOVÁ; Jana SKŘIČKOVÁ a František FORET. Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis. Journal of Chromatography A. AMSTERDAM: Elsevier, 2014, roč. 1358, September, s. 293-298. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2014.06.091.

@article{1205561,
   author = {Kubáň, Petr and Greguš, Michal and Pokojová, Eva and Skřičková, Jana and Foret, František},
   article_location = {AMSTERDAM},
   article_number = {September},
   doi = {https://doi.org/10.1016/j.chroma.2014.06.091},
   keywords = {Capillary electrophoresis; Contactless conductivity detection; Ions; Cystic fibrosis; Principal component analysis},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis},
   url = {http://www.sciencedirect.com/science/article/pii/S002196731401036X},
   volume = {1358},
   year = {2014}
}

TY  - JOUR
ID  - 1205561
AU  - Kubáň, Petr - Greguš, Michal - Pokojová, Eva - Skřičková, Jana - Foret, František
PY  - 2014
TI  - Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
JF  - Journal of Chromatography A
VL  - 1358
IS  - September
SP  - 293-298
EP  - 293-298
PB  - Elsevier
SN  - 00219673
KW  - Capillary electrophoresis
KW  - Contactless conductivity detection
KW  - Ions
KW  - Cystic fibrosis
KW  - Principal component analysis
UR  - http://www.sciencedirect.com/science/article/pii/S002196731401036X
L2  - http://www.sciencedirect.com/science/article/pii/S002196731401036X
N2  - A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed. (C) 2014 Elsevier B.V. All rights reserved.
ER  -

KUBÁŇ, Petr; Michal GREGUŠ; Eva POKOJOVÁ; Jana SKŘIČKOVÁ a František FORET. Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis. \textit{Journal of Chromatography A}. AMSTERDAM: Elsevier, 2014, roč.~1358, September, s.~293-298. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2014.06.091.

Přehled o publikaci