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@inproceedings{1206163, author = {Rozman, Tomáš and Farka, Zdeněk and Havliš, Jan and Lubal, Přemysl and Farková, Marta}, address = {Brno}, booktitle = {CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}, editor = {František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník}, keywords = {ANN; biogenic amines; dansyl chloride; HPLC}, howpublished = {elektronická verze "online"}, language = {eng}, location = {Brno}, isbn = {978-80-904959-2-0}, pages = {377-380}, publisher = {Institute of Analytical Chemistry AS CR}, title = {Use of artificial neural networks for optimization of biogenic amines derivatization}, url = {http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf#page=378}, year = {2014} }
TY - JOUR ID - 1206163 AU - Rozman, Tomáš - Farka, Zdeněk - Havliš, Jan - Lubal, Přemysl - Farková, Marta PY - 2014 TI - Use of artificial neural networks for optimization of biogenic amines derivatization PB - Institute of Analytical Chemistry AS CR CY - Brno SN - 9788090495920 KW - ANN KW - biogenic amines KW - dansyl chloride KW - HPLC UR - http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf#page=378 N2 - Biogenic amines as biologically active molecules with potential toxicological dangers are often subject of interest in food analysis. Since these substances do not exhibit significant fluorescence nor absorbance, derivatization is necessary for analysis in combination with RP-HPLC-UV setup. The use of experimental design (ED) in combination with artificial neural networks (ANN) for optimization of dansyl derivatization conditions for seven biogenic amines is presented. Four key factors were studied – concentration of derivatization reagent, buffer pH, temperature and incubation time. With the use of ED-ANN approach, optimal conditions for dansyl chloride derivatization were found and experimentally confirmed. ER -
ROZMAN, Tomáš, Zdeněk FARKA, Jan HAVLIŠ, Přemysl LUBAL and Marta FARKOVÁ. Use of artificial neural networks for optimization of biogenic amines derivatization. Online. In František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník. \textit{CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. Brno: Institute of Analytical Chemistry AS CR, 2014, p.~377-380. ISBN~978-80-904959-2-0.
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