In-Cell NMR and EPR Spectroscopy of Biomacromolecules

J 2014

In-Cell NMR and EPR Spectroscopy of Biomacromolecules

HANSEL, Robert, Laura M. LUH, Ivan CORBERSKI, Lukáš TRANTÍREK, Volker DOTSCH et. al.

Základní údaje

Originální název

In-Cell NMR and EPR Spectroscopy of Biomacromolecules

Autoři

HANSEL, Robert (276 Německo), Laura M. LUH (276 Německo), Ivan CORBERSKI (276 Německo), Lukáš TRANTÍREK (203 Česká republika, garant, domácí) a Volker DOTSCH (276 Německo)

Vydání

Angewandte Chemie International Edition, Weinheim, Germany, Wiley-VCH Verlag GmbH & Co. 2014, 1433-7851

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.261

Kód RIV

RIV/00216224:14740/14:00074124

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/anie.201311320

UT WoS

000342760700004

Klíčová slova anglicky

MAGNETIC-RESONANCE-SPECTROSCOPY; XENOPUS-LAEVIS OOCYTES; ELECTRON-PARAMAGNETIC-RESONANCE; PROTEIN-PROTEIN INTERACTIONS; HUMAN TELOMERIC DNA; DISTANCE MEASUREMENTS; ESCHERICHIA-COLI; LIVING CELLS; F-19 NMR; PHYSIOLOGICAL CONDITIONS

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 11. 2014 06:55, Martina Prášilová

Anotace

V originále

The dream of cell biologists is to be able to watch biological macromolecules perform their duties in the intracellular environment of live cells. Ideally, the observation of both the location and the conformation of these macromolecules with biophysical techniques is desired. The development of many fluorescence techniques, including super-resolution fluorescence microscopy, has significantly enhanced our ability to spot proteins and other molecules in the crowded cellular environment. However, the observation of their structure and conformational changes while they attend their business is still very challenging. In principle, NMR and EPR spectroscopy can be used to investigate the conformation and dynamics of biological macromolecules in living cells. The development of in-cell magnetic resonance techniques has demonstrated the feasibility of this approach. Herein we review the different techniques with a focus on liquid-state in-cell NMR spectroscopy, provide an overview of applications, and discuss the challenges that lie ahead.

Návaznosti

GA13-28310S, projekt VaV

Název: Evolučně konzervované strukturní vlastnosti centromerické a telomerické DNA

Investor: Grantová agentura ČR, Evolucne konzervované strukturní vlastnosti centromerické a telomerické DNA

Citovat

HANSEL, Robert, Laura M. LUH, Ivan CORBERSKI, Lukáš TRANTÍREK a Volker DOTSCH. In-Cell NMR and EPR Spectroscopy of Biomacromolecules. Angewandte Chemie International Edition. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co., 2014, roč. 53, č. 39, s. 10300-10314. ISSN 1433-7851. Dostupné z: https://dx.doi.org/10.1002/anie.201311320.

@article{1209991,
   author = {Hansel, Robert and Luh, Laura M. and Corberski, Ivan and Trantírek, Lukáš and Dotsch, Volker},
   article_location = {Weinheim, Germany},
   article_number = {39},
   doi = {http://dx.doi.org/10.1002/anie.201311320},
   keywords = {MAGNETIC-RESONANCE-SPECTROSCOPY; XENOPUS-LAEVIS OOCYTES; ELECTRON-PARAMAGNETIC-RESONANCE; PROTEIN-PROTEIN INTERACTIONS; HUMAN TELOMERIC DNA; DISTANCE MEASUREMENTS; ESCHERICHIA-COLI; LIVING CELLS; F-19 NMR; PHYSIOLOGICAL CONDITIONS},
   language = {eng},
   issn = {1433-7851},
   journal = {Angewandte Chemie International Edition},
   title = {In-Cell NMR and EPR Spectroscopy of Biomacromolecules},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract},
   volume = {53},
   year = {2014}
}

TY  - JOUR
ID  - 1209991
AU  - Hansel, Robert - Luh, Laura M. - Corberski, Ivan - Trantírek, Lukáš - Dotsch, Volker
PY  - 2014
TI  - In-Cell NMR and EPR Spectroscopy of Biomacromolecules
JF  - Angewandte Chemie International Edition
VL  - 53
IS  - 39
SP  - 10300-10314
EP  - 10300-10314
PB  - Wiley-VCH Verlag GmbH & Co.
SN  - 14337851
KW  - MAGNETIC-RESONANCE-SPECTROSCOPY
KW  - XENOPUS-LAEVIS OOCYTES
KW  - ELECTRON-PARAMAGNETIC-RESONANCE
KW  - PROTEIN-PROTEIN INTERACTIONS
KW  - HUMAN TELOMERIC DNA
KW  - DISTANCE MEASUREMENTS
KW  - ESCHERICHIA-COLI
KW  - LIVING CELLS
KW  - F-19 NMR
KW  - PHYSIOLOGICAL CONDITIONS
UR  - http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract
N2  - The dream of cell biologists is to be able to watch biological macromolecules perform their duties in the intracellular environment of live cells. Ideally, the observation of both the location and the conformation of these macromolecules with biophysical techniques is desired. The development of many fluorescence techniques, including super-resolution fluorescence microscopy, has significantly enhanced our ability to spot proteins and other molecules in the crowded cellular environment. However, the observation of their structure and conformational changes while they attend their business is still very challenging. In principle, NMR and EPR spectroscopy can be used to investigate the conformation and dynamics of biological macromolecules in living cells. The development of in-cell magnetic resonance techniques has demonstrated the feasibility of this approach. Herein we review the different techniques with a focus on liquid-state in-cell NMR spectroscopy, provide an overview of applications, and discuss the challenges that lie ahead.
ER  -

HANSEL, Robert, Laura M. LUH, Ivan CORBERSKI, Lukáš TRANTÍREK a Volker DOTSCH. In-Cell NMR and EPR Spectroscopy of Biomacromolecules. \textit{Angewandte Chemie International Edition}. Weinheim, Germany: Wiley-VCH Verlag GmbH \&{} Co., 2014, roč.~53, č.~39, s.~10300-10314. ISSN~1433-7851. Dostupné z: https://dx.doi.org/10.1002/anie.201311320.

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