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@article{1210516, author = {Vu, Giang T.H. and Cao, Hieu X. and Watanabe, Koichi and Hensel, Goetze and Blattner, Frank R. and Kumlehn, Jochen and Schubert, Ingo}, article_location = {Rockville (USA)}, article_number = {5}, doi = {http://dx.doi.org/10.1105/tpc.114.126607}, keywords = {SOMATIC PLANT-CELLS; HOMOLOGOUS RECOMBINATION; PHYSCOMITRELLA-PATENS; GENOME EVOLUTION; SEQUENCES; EXCHANGES; MECHANISM; REPLICATION; CONVERSION; CHOICE}, language = {eng}, issn = {1040-4651}, journal = {The Plant Cell}, title = {Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid}, url = {http://www.plantcell.org/content/26/5/2156}, volume = {26}, year = {2014} }
TY - JOUR ID - 1210516 AU - Vu, Giang T.H. - Cao, Hieu X. - Watanabe, Koichi - Hensel, Goetze - Blattner, Frank R. - Kumlehn, Jochen - Schubert, Ingo PY - 2014 TI - Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid JF - The Plant Cell VL - 26 IS - 5 SP - 2156-2167 EP - 2156-2167 PB - American Society of Plant Physiologists SN - 10404651 KW - SOMATIC PLANT-CELLS KW - HOMOLOGOUS RECOMBINATION KW - PHYSCOMITRELLA-PATENS KW - GENOME EVOLUTION KW - SEQUENCES KW - EXCHANGES KW - MECHANISM KW - REPLICATION KW - CONVERSION KW - CHOICE UR - http://www.plantcell.org/content/26/5/2156 L2 - http://www.plantcell.org/content/26/5/2156 N2 - DNA double-strand break (DSB) repair mechanisms differ in their requirements for a homologous repair template and in the accuracy of the result. We aimed to quantify the outcome of repair of a single targeted DSB in somatic cells of young barley (Hordeum vulgare) plants. Amplicon sequencing of three reporter constructs revealed 47 to 58% of reads as repaired via nonhomologous end-joining (NHEJ) with deletions and/or small (1 to 3 bp) insertions. Alternative NHEJ revealed 2 to 5 bp microhomology (15.7% of cases) or new replication-mediated short duplications at sealed breaks. Although deletions outweigh insertions in barley, this bias was less pronounced and deleted sequences were shorter than in Arabidopsis thaliana. Between 17 and 33% of reads likely represent restoration of the original sequence. Depending on the construct, 20 to 33% of reads arose via gene conversion (homologous recombination). Remarkably, < 1 to > 8% of reads apparently display synthesis-dependent strand annealing linked with NHEJ, inserting 4 to 61 bp, mostly originating from the surrounding of breakpoints. Positional coincidence of > 81% of sister chromatid exchanges with target loci is unprecedented for higher eukaryotes and indicates that most repair events for staggered DSBs, at least in barley, involve the sister chromatid and occur during S or G2 phase of the cell cycle. ER -
VU, Giang T.H., Hieu X. CAO, Koichi WATANABE, Goetze HENSEL, Frank R. BLATTNER, Jochen KUMLEHN a Ingo SCHUBERT. Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid. \textit{The Plant Cell}. Rockville (USA): American Society of Plant Physiologists, 2014, roč.~26, č.~5, s.~2156-2167. ISSN~1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.114.126607.
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