Loading of the centromeric histone H3 variant during
meiosis-how does it differ from mitosis?

J 2014

Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?

SCHUBERT, Veit, Inna LERMONTOVA a Ingo SCHUBERT

Základní údaje

Originální název

Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?

Autoři

SCHUBERT, Veit (276 Německo), Inna LERMONTOVA (276 Německo) a Ingo SCHUBERT (276 Německo, garant, domácí)

Vydání

Chromosoma, New York, Springer, 2014, 0009-5915

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 4.602

Kód RIV

RIV/00216224:14740/14:00077480

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s00412-014-0466-9

UT WoS

000342465800008

Klíčová slova anglicky

CELL-CYCLE PROGRESSION; CENP-A; CHROMOSOME; DYNAMICS; HETEROCHROMATIN; SEGREGATION; REQUIRES; PLANTS

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 12. 2019 15:20, Mgr. Marie Šípková, DiS.

Anotace

V originále

In eukaryotic phyla studied so far, the essential centromeric histone H3 variant (CENH3) is loaded to centromeric nucleosomes after S-phase (except for yeast) but before mitotic segregation (except for metazoan). While the C-terminal part of CENH3 seems to be sufficient for mitotic centromere function in plants, meiotic centromeres neither load nor tolerate impaired CENH3 molecules. However, details about CENH3 deposition in meiocytes are unknown (except for Drosophila). Therefore, we quantified fluorescence signals after the immunostaining of CENH3 along meiotic and mitotic nuclear division cycles of rye, a monocotyledonous plant. One peak of fluorescence intensity appeared in the early meiotic prophase of pollen mother cells and a second one during interkinesis, both followed by a decrease of CENH3. Then, the next loading occurred in the male gametophyte before its first mitotic division. These data indicate that CENH3 loading differs between mitotic and meiotic nuclei. Contrary to the situation in mitotic cycles, CENH3 deposition is biphasic during meiosis and apparently linked with a quality check, a removal of impaired CENH3 molecules, and a general loss of CENH3 after each loading phase. These steps ensure an endowment of centromeres with a sufficient amount of correct CENH3 molecules as a prerequisite for centromere maintenance during mitotic cycles of the microgametophyte and the progeny. From a comparison with data available for Drosophila, we hypothesise that the post-divisional mitotic CENH3 loading in metazoans is evolutionarily derived from the post-divisional meiotic loading phase, while the pre-divisional first meiotic loading has been conserved among eukaryotes.

Návaznosti

EE2.3.20.0189, projekt VaV

Název: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

Citovat

SCHUBERT, Veit, Inna LERMONTOVA a Ingo SCHUBERT. Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis? Chromosoma. New York: Springer, 2014, roč. 123, č. 5, s. 491-497. ISSN 0009-5915. Dostupné z: https://dx.doi.org/10.1007/s00412-014-0466-9.

@article{1210518,
   author = {Schubert, Veit and Lermontova, Inna and Schubert, Ingo},
   article_location = {New York},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00412-014-0466-9},
   keywords = {CELL-CYCLE PROGRESSION; CENP-A; CHROMOSOME; DYNAMICS; HETEROCHROMATIN; SEGREGATION; REQUIRES; PLANTS},
   language = {eng},
   issn = {0009-5915},
   journal = {Chromosoma},
   title = {Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/24806806},
   volume = {123},
   year = {2014}
}

TY  - JOUR
ID  - 1210518
AU  - Schubert, Veit - Lermontova, Inna - Schubert, Ingo
PY  - 2014
TI  - Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?
JF  - Chromosoma
VL  - 123
IS  - 5
SP  - 491-497
EP  - 491-497
PB  - Springer
SN  - 00095915
KW  - CELL-CYCLE PROGRESSION
KW  - CENP-A
KW  - CHROMOSOME
KW  - DYNAMICS
KW  - HETEROCHROMATIN
KW  - SEGREGATION
KW  - REQUIRES
KW  - PLANTS
UR  - http://www.ncbi.nlm.nih.gov/pubmed/24806806
L2  - http://www.ncbi.nlm.nih.gov/pubmed/24806806
N2  - In eukaryotic phyla studied so far, the essential centromeric histone H3 variant (CENH3) is loaded to centromeric nucleosomes after S-phase (except for yeast) but before mitotic segregation (except for metazoan). While the C-terminal part of CENH3 seems to be sufficient for mitotic centromere function in plants, meiotic centromeres neither load nor tolerate impaired CENH3 molecules. However, details about CENH3 deposition in meiocytes are unknown (except for Drosophila). Therefore, we quantified fluorescence signals after the immunostaining of CENH3 along meiotic and mitotic nuclear division cycles of rye, a monocotyledonous plant. One peak of fluorescence intensity appeared in the early meiotic prophase of pollen mother cells and a second one during interkinesis, both followed by a decrease of CENH3. Then, the next loading occurred in the male gametophyte before its first mitotic division. These data indicate that CENH3 loading differs between mitotic and meiotic nuclei. Contrary to the situation in mitotic cycles, CENH3 deposition is biphasic during meiosis and apparently linked with a quality check, a removal of impaired CENH3 molecules, and a general loss of CENH3 after each loading phase. These steps ensure an endowment of centromeres with a sufficient amount of correct CENH3 molecules as a prerequisite for centromere maintenance during mitotic cycles of the microgametophyte and the progeny. From a comparison with data available for Drosophila, we hypothesise that the post-divisional mitotic CENH3 loading in metazoans is evolutionarily derived from the post-divisional meiotic loading phase, while the pre-divisional first meiotic loading has been conserved among eukaryotes.
ER  -

SCHUBERT, Veit, Inna LERMONTOVA a Ingo SCHUBERT. Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis? \textit{Chromosoma}. New York: Springer, 2014, roč.~123, č.~5, s.~491-497. ISSN~0009-5915. Dostupné z: https://dx.doi.org/10.1007/s00412-014-0466-9.

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