When fathers are instant losers: homogenization of rDNA loci in
recently formed Cardamine X schulzii trigenomic allopolyploid

J 2014

When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

ZOZOMOVÁ-LIHOVÁ, Judita, Terezie MANDÁKOVÁ, Alena KOVAŘÍKOVÁ, Andreas MÜHLHAUSEN, Klaus MUMMENHOFF et. al.

Základní údaje

Originální název

When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

Autoři

ZOZOMOVÁ-LIHOVÁ, Judita (703 Slovensko), Terezie MANDÁKOVÁ (203 Česká republika, domácí), Alena KOVAŘÍKOVÁ (203 Česká republika), Andreas MÜHLHAUSEN (276 Německo), Klaus MUMMENHOFF (276 Německo), Martin LYSÁK (203 Česká republika, garant, domácí) a Aleš KOVAŘÍK (203 Česká republika)

Vydání

New Phytologist, Hoboken, WILEY-BLACKWELL, 2014, 0028-646X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.672

Kód RIV

RIV/00216224:14740/14:00074135

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/nph.12873

UT WoS

000340286000010

Klíčová slova anglicky

NUCLEAR RIBOSOMAL DNA; RNA GENE SITES; CONCERTED EVOLUTION; TRAGOPOGON ASTERACEAE; NATURAL-POPULATIONS; SENECIO-CAMBRENSIS; MOLECULAR EVIDENCE; BRASSICA-NAPUS; PARENTAL RDNA; GENOME

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2015 13:49, Martina Prášilová

Anotace

V originále

Recently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35S rRNA genes (rDNA) in the Cardamine x schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. x insueta triploid hybrid derived from C. rivularis (female) and C. amara (male) had gene ratios highly skewed towards maternal sequences. Similarly, C. x schulzii, originating from the secondary hybridization event involving C. x insueta (female) and C. pratensis (male), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. x schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes. Prevalent clonal propagation of allopolyploids, C. x insueta and C. x schulzii, indicates that concerted evolution of rDNA may occur in the absence of extensive meiotic cycles. Adoption of NGS in rDNA variant analysis is highly informative for deciphering the evolutionary histories of allopolyploid species with ongoing homogenization processes.

Návaznosti

EE2.3.20.0189, projekt VaV

Název: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

EE2.3.30.0037, projekt VaV

Název: Zaměstnáním nejlepších mladých vědců k rozvoji mezinárodní spolupráce

GBP501/12/G090, projekt VaV

Název: Evoluce a funkce komplexních genomů rostlin

Citovat

ZOZOMOVÁ-LIHOVÁ, Judita, Terezie MANDÁKOVÁ, Alena KOVAŘÍKOVÁ, Andreas MÜHLHAUSEN, Klaus MUMMENHOFF, Martin LYSÁK a Aleš KOVAŘÍK. When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid. New Phytologist. Hoboken: WILEY-BLACKWELL, 2014, roč. 203, č. 4, s. 1096-1108. ISSN 0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.12873.

@article{1210545,
   author = {ZozomováandLihová, Judita and Mandáková, Terezie and Kovaříková, Alena and Mühlhausen, Andreas and Mummenhoff, Klaus and Lysák, Martin and Kovařík, Aleš},
   article_location = {Hoboken},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/nph.12873},
   keywords = {NUCLEAR RIBOSOMAL DNA; RNA GENE SITES; CONCERTED EVOLUTION; TRAGOPOGON ASTERACEAE; NATURAL-POPULATIONS; SENECIO-CAMBRENSIS; MOLECULAR EVIDENCE; BRASSICA-NAPUS; PARENTAL RDNA; GENOME},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf},
   volume = {203},
   year = {2014}
}

TY  - JOUR
ID  - 1210545
AU  - Zozomová-Lihová, Judita - Mandáková, Terezie - Kovaříková, Alena - Mühlhausen, Andreas - Mummenhoff, Klaus - Lysák, Martin - Kovařík, Aleš
PY  - 2014
TI  - When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid
JF  - New Phytologist
VL  - 203
IS  - 4
SP  - 1096-1108
EP  - 1096-1108
PB  - WILEY-BLACKWELL
SN  - 0028646X
KW  - NUCLEAR RIBOSOMAL DNA
KW  - RNA GENE SITES
KW  - CONCERTED EVOLUTION
KW  - TRAGOPOGON ASTERACEAE
KW  - NATURAL-POPULATIONS
KW  - SENECIO-CAMBRENSIS
KW  - MOLECULAR EVIDENCE
KW  - BRASSICA-NAPUS
KW  - PARENTAL RDNA
KW  - GENOME
UR  - http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf
L2  - http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf
N2  - Recently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35S rRNA genes (rDNA) in the Cardamine x schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. x insueta triploid hybrid derived from C. rivularis (female) and C. amara (male) had gene ratios highly skewed towards maternal sequences. Similarly, C. x schulzii, originating from the secondary hybridization event involving C. x insueta (female) and C. pratensis (male), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. x schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes. Prevalent clonal propagation of allopolyploids, C. x insueta and C. x schulzii, indicates that concerted evolution of rDNA may occur in the absence of extensive meiotic cycles. Adoption of NGS in rDNA variant analysis is highly informative for deciphering the evolutionary histories of allopolyploid species with ongoing homogenization processes.
ER  -

ZOZOMOVÁ-LIHOVÁ, Judita, Terezie MANDÁKOVÁ, Alena KOVAŘÍKOVÁ, Andreas MÜHLHAUSEN, Klaus MUMMENHOFF, Martin LYSÁK a Aleš KOVAŘÍK. When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid. \textit{New Phytologist}. Hoboken: WILEY-BLACKWELL, 2014, roč.~203, č.~4, s.~1096-1108. ISSN~0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.12873.

Podrobný výpis o publikaci