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@inproceedings{1210572, author = {Schejbal, Jan and Řemínek, Roman and Glatz, Zdeněk}, address = {Brno}, booktitle = {11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014}, editor = {F.Foret}, keywords = {kapilární elektroforéza; metabolismus léčiv; cytochrom P450 2C9; IMER; diclofenac; sulfafenazol; kinetika; inhibice;}, howpublished = {paměťový nosič}, language = {eng}, location = {Brno}, isbn = {978-80-904959-2-0}, pages = {103-106}, publisher = {Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika}, title = {TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION}, year = {2014} }
TY - JOUR ID - 1210572 AU - Schejbal, Jan - Řemínek, Roman - Glatz, Zdeněk PY - 2014 TI - TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION PB - Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika CY - Brno SN - 9788090495920 KW - kapilární elektroforéza KW - metabolismus léčiv KW - cytochrom P450 2C9 KW - IMER KW - diclofenac KW - sulfafenazol KW - kinetika KW - inhibice; N2 - The enzyme superfamily of cytochromes P450 (CYP) plays a key role in xenobiotic biotransformation and thusly in drug metabolism. Therefore assessment of the interaction between candidate drugs and CYP isoforms is an integral part of preclinical stage of the drug development process, where high throughput and cost effectiveness is a priority. Capillary electrophoresis (CE) offers a number of features which are suitable for these purposes, those being the low sample consumption, short analysis time and parallel analyses using multicapillary systems, what more integration of immobilized enzyme microreactor (IMER) into the separation capillary enables full automation and repeated use of the enzyme. A new online method for inhibition studies based on cytochrome P450 2C9 isoform IMER integrated into CE system is thus presented. ER -
SCHEJBAL, Jan, Roman ŘEMÍNEK a Zdeněk GLATZ. TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION. In F.Foret. \textit{11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014}. Brno: Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika, 2014, s.~103-106. ISBN~978-80-904959-2-0.
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