| LEXA, Matej, Pavlina STEFLOVA, Tomáš MARTÍNEK, Michaela VORLÍČKOVÁ, Boris VYSKOT a Eduard KEJNOVSKÝ. Guanine quadruplexes are formed by specific regions of human transposable elements. BMC Genomics. London: BioMed Central Ltd, 2014, roč. 15, č. 1, s. 1032-1043. ISSN 1471-2164. Dostupné z: https://dx.doi.org/10.1186/1471-2164-15-1032. |
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@article{1210725,
author = {Lexa, Matej and Steflova, Pavlina and Martínek, Tomáš and Vorlíčková, Michaela and Vyskot, Boris and Kejnovský, Eduard},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1186/1471-2164-15-1032},
keywords = {G4 quadruplex; human transposable elements; circular dichroism; Bioconductor code; bioinformatics},
language = {eng},
issn = {1471-2164},
journal = {BMC Genomics},
title = {Guanine quadruplexes are formed by specific regions of human transposable elements},
url = {http://www.biomedcentral.com/1471-2164/15/1032},
volume = {15},
year = {2014}
}
TY - JOUR
ID - 1210725
AU - Lexa, Matej - Steflova, Pavlina - Martínek, Tomáš - Vorlíčková, Michaela - Vyskot, Boris - Kejnovský, Eduard
PY - 2014
TI - Guanine quadruplexes are formed by specific regions of human transposable elements
JF - BMC Genomics
VL - 15
IS - 1
SP - 1032
EP - 1032
PB - BioMed Central Ltd
SN - 14712164
KW - G4 quadruplex
KW - human transposable elements
KW - circular dichroism
KW - Bioconductor code
KW - bioinformatics
UR - http://www.biomedcentral.com/1471-2164/15/1032
N2 - BACKGROUND:Transposable elements form a significant proportion of eukaryotic genomes. Recently, Lexa et al (Nucleic Acids Res 42:968-978, 2014) reported that plant long terminal repeat (LTR) retrotransposons often contain potential quadruplex sequences (PQSs) in their LTRs and experimentally confirmed their ability to adopt four-stranded DNA conformations.RESULTS:Here, we searched for PQSs in human retrotransposons and found that PQSs are specifically localized in the 3'-UTR of LINE-1 elements, in LTRs of HERV elements and are strongly accumulated in specific regions of SVA elements. Circular dichroism spectroscopy confirmed that most PQSs had adopted monomolecular or bimolecular guanine quadruplex structures. Evolutionarily young SVA elements contained more PQSs than older elements and their propensity to form quadruplex DNA was higher. Full-length L1 elements contained more PQSs than truncated elements; the highest proportion of PQSs was found inside transpositionally active L1 elements (PA2 and HS families).CONCLUSIONS:Conservation of quadruplexes at specific positions of transposable elements implies their importance in their life cycle. The increasing quadruplex presence in evolutionary young LINE-1 and SVA families makes these elements important contributors toward present genome-wide quadruplex distribution.
ER -
LEXA, Matej, Pavlina STEFLOVA, Tomáš MARTÍNEK, Michaela VORLÍČKOVÁ, Boris VYSKOT a Eduard KEJNOVSKÝ. Guanine quadruplexes are formed by specific regions of human transposable elements. \textit{BMC Genomics}. London: BioMed Central Ltd, 2014, roč.~15, č.~1, s.~1032-1043. ISSN~1471-2164. Dostupné z: https://dx.doi.org/10.1186/1471-2164-15-1032.
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