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@inproceedings{1210760,
author = {Civáňová, Kristína},
address = {Brno},
booktitle = {3rd Workshop of European Centre of Ichthyoparasitology},
editor = {Šárka Mašová, Milan Gelnar},
keywords = {MHC; gene expression; HRM; Cyprinidae},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Brno},
isbn = {978-80-210-7492-7},
pages = {10-11},
publisher = {MUNI Press},
title = {Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?},
year = {2014}
}
TY - JOUR
ID - 1210760
AU - Civáňová, Kristína
PY - 2014
TI - Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?
PB - MUNI Press
CY - Brno
SN - 9788021074927
KW - MHC
KW - gene expression
KW - HRM
KW - Cyprinidae
N2 - We have confirmed extremely high level of allelic diversity in several fish genera by classical genotyping. We used two basic approaches for this purpose. First, the older, was CE-SSCP (Capillary Electrophoresis-Single Stranded Conformational Polymorphism) of PCR amplicon clones combined with automatic sequencing based on Sanger technology (ABI 3130 Genetic Analyzer). The second, modern, was 454 clonal pyrosequencing of heterozygous amplicons (454 GS FLX platform with Titanium chemistry). Recently, using CFX96 real time PCR system, we test also the expression levels of fish MHC genes (DAB1 and DAB3) by quantitative real-time PCR (qPCR) and we launched a new approach for MHC genes variability tests - high resolution melting analysis (HRM), too. After completing "HRM allele profile database" and finish analyses of qPCR we will be exploring associations with parasite load in chosen Cyprinid species.
ER -
CIVÁŇOVÁ, Kristína. Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence? In Šárka Mašová, Milan Gelnar. \textit{3rd Workshop of European Centre of Ichthyoparasitology}. Brno: MUNI Press, 2014, s.~10-11. ISBN~978-80-210-7492-7.
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