| KŘIVÁNEK, Jan, Eva ŠVANDOVÁ, Jan KRÁLIK, Šimon HAJDA, Radek FEDR, Vladimír VINARSKÝ, Josef JAROŠ, K. SOUČEK, M. BUCHTOVÁ, Eva MATALOVÁ and Aleš HAMPL. Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells. Folia biologica. Prague: Charles University, 2014, vol. 60, Suppl. 1, p. 30-38. ISSN 0015-5500. |
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@article{1212374,
author = {Křivánek, Jan and Švandová, Eva and Králik, Jan and Hajda, Šimon and Fedr, Radek and Vinarský, Vladimír and Jaroš, Josef and Souček, K. and Buchtová, M. and Matalová, Eva and Hampl, Aleš},
article_location = {Prague},
article_number = {Suppl. 1},
keywords = {stem cell differentiation; neural crest; odontogenesis; cell therapies},
language = {eng},
issn = {0015-5500},
journal = {Folia biologica},
title = {Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells},
volume = {60},
year = {2014}
}
TY - JOUR
ID - 1212374
AU - Křivánek, Jan - Švandová, Eva - Králik, Jan - Hajda, Šimon - Fedr, Radek - Vinarský, Vladimír - Jaroš, Josef - Souček, K. - Buchtová, M. - Matalová, Eva - Hampl, Aleš
PY - 2014
TI - Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells
JF - Folia biologica
VL - 60
IS - Suppl. 1
SP - 30-38
EP - 30-38
PB - Charles University
SN - 00155500
KW - stem cell differentiation
KW - neural crest
KW - odontogenesis
KW - cell therapies
N2 - Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to their multilineage differentiation capacity NCCs offer a cell source for regenerative medicine. Both these aspects make NCC biology an important issue to study, which can currently be addressed using methodologies based on pluripotent stem cells. Here we contributed to understanding the biology of human NCCs by refining the protocol for differentiation/propagation of NCC-like cells from human embryonic stem cells and by characterizing the molecular and functional phenotype of such cells. Most importantly, we improved formulation of media for NCC culture, we found that poly-L-ornithine combined with fibronectin provide good support for NCC growth, we unravelled the tendency of cultured NCCs to maintain heterogeneity of CD271 expression, and we showed that NCCs derived here possess the capacity to react to BMP4 signals by dramatically up-regulating MSX1, which is linked to odontogenesis.
ER -
KŘIVÁNEK, Jan, Eva ŠVANDOVÁ, Jan KRÁLIK, Šimon HAJDA, Radek FEDR, Vladimír VINARSKÝ, Josef JAROŠ, K. SOUČEK, M. BUCHTOVÁ, Eva MATALOVÁ and Aleš HAMPL. Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells. \textit{Folia biologica}. Prague: Charles University, 2014, vol.~60, Suppl. 1, p.~30-38. ISSN~0015-5500.
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