Stepwise Enhancement of Catalytic Performance of Haloalkane
Dehalogenase LinB towards Beta-Hexachlorocyclohexane.

J 2014

Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.

MORIUCHI, Ryota, Hiroki TANAKA, Yuki NIKAWADORI, Mayuko ISHITSUKA, Michihiro ITO et. al.

Základní údaje

Originální název

Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.

Autoři

MORIUCHI, Ryota (392 Japonsko), Hiroki TANAKA (392 Japonsko), Yuki NIKAWADORI (392 Japonsko), Mayuko ISHITSUKA (392 Japonsko), Michihiro ITO (392 Japonsko), Yoshiyuki OHTSUBO (392 Japonsko), Masataka TSUDA (392 Japonsko), Jiří DAMBORSKÝ (203 Česká republika, garant, domácí), Zbyněk PROKOP (203 Česká republika, domácí) a Yuji NAGATA (392 Japonsko)

Vydání

Applied Microbiology and Biotechnology Express, London, Springer, 2014, 2191-0855

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Kód RIV

RIV/00216224:14310/14:00074201

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1186/s13568-014-0072-5

UT WoS

000358066200001

Klíčová slova anglicky

beta -Hexachlorocyclohexane; Xenobiotics; Biodegradation; Haloalkane dehalogenase; Protein evolution

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 12. 2019 09:14, Mgr. Marie Šípková, DiS.

Anotace

V originále

Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid (aa) residues, exhibit only seven amino acid residue differences between them, but LinBMI‘s catalytic performance towards beta-hexachlorocyclohexane (beta-HCH) is considerably higher than LinBUT‘s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT‘s b-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations.

Návaznosti

GAP503/12/0572, projekt VaV

Název: Konstrukce syntetické metabolické dráhy pro degradaci důležitého environmentálního polutantu proteinovým a metabolickým inženýrstvím

Investor: Grantová agentura ČR, Konstrukce syntetické metabolické dráhy pro degradaci důležitého environmentlního polutantu proteinovým a metabolickým inženýrstvím

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

MORIUCHI, Ryota, Hiroki TANAKA, Yuki NIKAWADORI, Mayuko ISHITSUKA, Michihiro ITO, Yoshiyuki OHTSUBO, Masataka TSUDA, Jiří DAMBORSKÝ, Zbyněk PROKOP a Yuji NAGATA. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. Applied Microbiology and Biotechnology Express. London: Springer, 2014, roč. 4, č. 72, s. 1-10. ISSN 2191-0855. Dostupné z: https://dx.doi.org/10.1186/s13568-014-0072-5.

@article{1213423,
   author = {Moriuchi, Ryota and Tanaka, Hiroki and Nikawadori, Yuki and Ishitsuka, Mayuko and Ito, Michihiro and Ohtsubo, Yoshiyuki and Tsuda, Masataka and Damborský, Jiří and Prokop, Zbyněk and Nagata, Yuji},
   article_location = {London},
   article_number = {72},
   doi = {http://dx.doi.org/10.1186/s13568-014-0072-5},
   keywords = {beta -Hexachlorocyclohexane; Xenobiotics; Biodegradation; Haloalkane dehalogenase; Protein evolution},
   language = {eng},
   issn = {2191-0855},
   journal = {Applied Microbiology and Biotechnology Express},
   title = {Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.},
   url = {https://amb-express.springeropen.com/track/pdf/10.1186/s13568-014-0072-5},
   volume = {4},
   year = {2014}
}

TY  - JOUR
ID  - 1213423
AU  - Moriuchi, Ryota - Tanaka, Hiroki - Nikawadori, Yuki - Ishitsuka, Mayuko - Ito, Michihiro - Ohtsubo, Yoshiyuki - Tsuda, Masataka - Damborský, Jiří - Prokop, Zbyněk - Nagata, Yuji
PY  - 2014
TI  - Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.
JF  - Applied Microbiology and Biotechnology Express
VL  - 4
IS  - 72
SP  - 1-10
EP  - 1-10
PB  - Springer
SN  - 21910855
KW  - beta -Hexachlorocyclohexane
KW  - Xenobiotics
KW  - Biodegradation
KW  - Haloalkane dehalogenase
KW  - Protein evolution
UR  - https://amb-express.springeropen.com/track/pdf/10.1186/s13568-014-0072-5
L2  - https://amb-express.springeropen.com/track/pdf/10.1186/s13568-014-0072-5
N2  - Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid (aa) residues, exhibit only seven amino acid residue differences between them, but LinBMI‘s catalytic performance towards beta-hexachlorocyclohexane (beta-HCH) is considerably higher than LinBUT‘s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT‘s b-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations.
ER  -

MORIUCHI, Ryota, Hiroki TANAKA, Yuki NIKAWADORI, Mayuko ISHITSUKA, Michihiro ITO, Yoshiyuki OHTSUBO, Masataka TSUDA, Jiří DAMBORSKÝ, Zbyněk PROKOP a Yuji NAGATA. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. \textit{Applied Microbiology and Biotechnology Express}. London: Springer, 2014, roč.~4, č.~72, s.~1-10. ISSN~2191-0855. Dostupné z: https://dx.doi.org/10.1186/s13568-014-0072-5.

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