DVOŘÁK, Pavel, Nagendra Prasad KURUMBANG, Jaroslav BENDL, Jan BREZOVSKÝ, Zbyněk PROKOP a Jiří DAMBORSKÝ. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. ChemBioChem. WEINHEIM: WILEY-VCH, 2014, roč. 15, č. 13, s. 1891-1895. ISSN 1439-4227. Dostupné z: https://dx.doi.org/10.1002/cbic.201402265. |
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@article{1213428, author = {Dvořák, Pavel and Kurumbang, Nagendra Prasad and Bendl, Jaroslav and Brezovský, Jan and Prokop, Zbyněk and Damborský, Jiří}, article_location = {WEINHEIM}, article_number = {13}, doi = {http://dx.doi.org/10.1002/cbic.201402265}, keywords = {biocatalysis; engineered enzymes}, language = {eng}, issn = {1439-4227}, journal = {ChemBioChem}, title = {Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization.}, volume = {15}, year = {2014} }
TY - JOUR ID - 1213428 AU - Dvořák, Pavel - Kurumbang, Nagendra Prasad - Bendl, Jaroslav - Brezovský, Jan - Prokop, Zbyněk - Damborský, Jiří PY - 2014 TI - Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. JF - ChemBioChem VL - 15 IS - 13 SP - 1891-1895 EP - 1891-1895 PB - WILEY-VCH SN - 14394227 KW - biocatalysis KW - engineered enzymes N2 - Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts’ stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes. ER -
DVOŘÁK, Pavel, Nagendra Prasad KURUMBANG, Jaroslav BENDL, Jan BREZOVSKÝ, Zbyněk PROKOP a Jiří DAMBORSKÝ. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. \textit{ChemBioChem}. WEINHEIM: WILEY-VCH, 2014, roč.~15, č.~13, s.~1891-1895. ISSN~1439-4227. Dostupné z: https://dx.doi.org/10.1002/cbic.201402265.
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