Interfacing Microwells with Nanoliter Compartments: A Sampler
Generating High-Resolution Concentration Gradients for
Quantitative Biochemical Analyses in Droplets

J 2015

Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets

GIELEN, Fabrice; Tomáš BURYŠKA; Liisa VAN VLIET; Maren BUTZ; Jiří DAMBORSKÝ et. al.

Basic information

Original name

Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets

Authors

GIELEN, Fabrice (250 France); Tomáš BURYŠKA (203 Czech Republic, belonging to the institution); Liisa VAN VLIET (840 United States of America); Maren BUTZ (756 Switzerland); Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution); Zbyněk PROKOP (203 Czech Republic, belonging to the institution) and Florian HOLLFELDER (276 Germany)

Edition

Analytical Chemistry, 2015, 0003-2700

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 5.886

RIV identification code

RIV/00216224:14310/15:00082195

Organization unit

Faculty of Science

DOI

https://doi.org/10.1021/ac503336g

UT WoS

000347590400064

EID Scopus

2-s2.0-84920395903

Keywords in English

microfluidic; haloalkane dehalogenase DbjA

Abstract

In the original language

Analysis of concentration-dependencies is key for the quantitative understanding of biological and chemical systems. In experimental tests involving concentration gradients such as inhibitor library screening, the number of data points and the ratio between the stock volume and the volume required in each test determine the quality and efficiency of the information gained. Titerplate assays are currently the most widely used format, even though they require microlitre volumes. Compartmentalization of reactions in pico- to nanolitre water-in-oil droplets in microfluidic devices provides a solution for massive volume reduction. This work addresses the challenge to produce microfluidic-based concentration gradients in a way that every droplet represents one unique reagent combination. We present a simple microcapillary technique able to generate such series of monodisperse water-in-oil droplets (with frequency up to 10 Hz) from a sample presented in an open well, e.g. a titreplate. Time-dependent variation of the well content results in microdroplets that represent time capsules of the composition of the source well.

Links

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Cite

GIELEN, Fabrice; Tomáš BURYŠKA; Liisa VAN VLIET; Maren BUTZ; Jiří DAMBORSKÝ; Zbyněk PROKOP and Florian HOLLFELDER. Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets. Analytical Chemistry. 2015, vol. 87, No 1, p. 624-632. ISSN 0003-2700. Available from: https://doi.org/10.1021/ac503336g.

@article{1213476,
   author = {Gielen, Fabrice and Buryška, Tomáš and Van Vliet, Liisa and Butz, Maren and Damborský, Jiří and Prokop, Zbyněk and Hollfelder, Florian},
   article_number = {1},
   doi = {https://doi.org/10.1021/ac503336g},
   keywords = {microfluidic; haloalkane dehalogenase DbjA},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac503336g},
   volume = {87},
   year = {2015}
}

TY  - JOUR
ID  - 1213476
AU  - Gielen, Fabrice - Buryška, Tomáš - Van Vliet, Liisa - Butz, Maren - Damborský, Jiří - Prokop, Zbyněk - Hollfelder, Florian
PY  - 2015
TI  - Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets
JF  - Analytical Chemistry
VL  - 87
IS  - 1
SP  - 624-632
EP  - 624-632
SN  - 00032700
KW  - microfluidic
KW  - haloalkane dehalogenase DbjA
UR  - http://pubs.acs.org/doi/abs/10.1021/ac503336g
N2  - Analysis of concentration-dependencies is key for the quantitative understanding of biological and chemical systems. In experimental tests involving concentration gradients such as inhibitor library screening, the number of data points and the ratio between the stock volume and the volume required in each test determine the quality and efficiency of the information gained. Titerplate assays are currently the most widely used format, even though they require microlitre volumes. Compartmentalization of reactions in pico- to nanolitre water-in-oil droplets in microfluidic devices provides a solution for massive volume reduction. This work addresses the challenge to produce microfluidic-based concentration gradients in a way that every droplet represents one unique reagent combination. We present a simple microcapillary technique able to generate such series of monodisperse water-in-oil droplets (with frequency up to 10 Hz) from a sample presented in an open well, e.g. a titreplate. Time-dependent variation of the well content results in microdroplets that represent time capsules of the composition of the source well.
ER  -

GIELEN, Fabrice; Tomáš BURYŠKA; Liisa VAN VLIET; Maren BUTZ; Jiří DAMBORSKÝ; Zbyněk PROKOP and Florian HOLLFELDER. Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets. \textit{Analytical Chemistry}. 2015, vol.~87, No~1, p.~624-632. ISSN~0003-2700. Available from: https://doi.org/10.1021/ac503336g.

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