Interfacing Microwells with Nanoliter Compartments: A Sampler
Generating High-Resolution Concentration Gradients for
Quantitative Biochemical Analyses in Droplets

J 2015

Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets

GIELEN, Fabrice, Tomáš BURYŠKA, Liisa VAN VLIET, Maren BUTZ, Jiří DAMBORSKÝ et. al.

Základní údaje

Originální název

Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets

Autoři

GIELEN, Fabrice (250 Francie), Tomáš BURYŠKA (203 Česká republika, domácí), Liisa VAN VLIET (840 Spojené státy), Maren BUTZ (756 Švýcarsko), Jiří DAMBORSKÝ (203 Česká republika, garant, domácí), Zbyněk PROKOP (203 Česká republika, domácí) a Florian HOLLFELDER (276 Německo)

Vydání

Analytical Chemistry, 2015, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.886

Kód RIV

RIV/00216224:14310/15:00082195

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1021/ac503336g

UT WoS

000347590400064

Klíčová slova anglicky

microfluidic; haloalkane dehalogenase DbjA

Štítky

AKR, rivok

Změněno: 21. 3. 2017 07:50, prof. Mgr. Jiří Damborský, Dr.

Anotace

V originále

Analysis of concentration-dependencies is key for the quantitative understanding of biological and chemical systems. In experimental tests involving concentration gradients such as inhibitor library screening, the number of data points and the ratio between the stock volume and the volume required in each test determine the quality and efficiency of the information gained. Titerplate assays are currently the most widely used format, even though they require microlitre volumes. Compartmentalization of reactions in pico- to nanolitre water-in-oil droplets in microfluidic devices provides a solution for massive volume reduction. This work addresses the challenge to produce microfluidic-based concentration gradients in a way that every droplet represents one unique reagent combination. We present a simple microcapillary technique able to generate such series of monodisperse water-in-oil droplets (with frequency up to 10 Hz) from a sample presented in an open well, e.g. a titreplate. Time-dependent variation of the well content results in microdroplets that represent time capsules of the composition of the source well.

Návaznosti

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

GIELEN, Fabrice, Tomáš BURYŠKA, Liisa VAN VLIET, Maren BUTZ, Jiří DAMBORSKÝ, Zbyněk PROKOP a Florian HOLLFELDER. Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets. Analytical Chemistry. 2015, roč. 87, č. 1, s. 624-632. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/ac503336g.

@article{1213476,
   author = {Gielen, Fabrice and Buryška, Tomáš and Van Vliet, Liisa and Butz, Maren and Damborský, Jiří and Prokop, Zbyněk and Hollfelder, Florian},
   article_number = {1},
   doi = {http://dx.doi.org/10.1021/ac503336g},
   keywords = {microfluidic; haloalkane dehalogenase DbjA},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac503336g},
   volume = {87},
   year = {2015}
}

TY  - JOUR
ID  - 1213476
AU  - Gielen, Fabrice - Buryška, Tomáš - Van Vliet, Liisa - Butz, Maren - Damborský, Jiří - Prokop, Zbyněk - Hollfelder, Florian
PY  - 2015
TI  - Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets
JF  - Analytical Chemistry
VL  - 87
IS  - 1
SP  - 624-632
EP  - 624-632
SN  - 00032700
KW  - microfluidic
KW  - haloalkane dehalogenase DbjA
UR  - http://pubs.acs.org/doi/abs/10.1021/ac503336g
N2  - Analysis of concentration-dependencies is key for the quantitative understanding of biological and chemical systems. In experimental tests involving concentration gradients such as inhibitor library screening, the number of data points and the ratio between the stock volume and the volume required in each test determine the quality and efficiency of the information gained. Titerplate assays are currently the most widely used format, even though they require microlitre volumes. Compartmentalization of reactions in pico- to nanolitre water-in-oil droplets in microfluidic devices provides a solution for massive volume reduction. This work addresses the challenge to produce microfluidic-based concentration gradients in a way that every droplet represents one unique reagent combination. We present a simple microcapillary technique able to generate such series of monodisperse water-in-oil droplets (with frequency up to 10 Hz) from a sample presented in an open well, e.g. a titreplate. Time-dependent variation of the well content results in microdroplets that represent time capsules of the composition of the source well.
ER  -

GIELEN, Fabrice, Tomáš BURYŠKA, Liisa VAN VLIET, Maren BUTZ, Jiří DAMBORSKÝ, Zbyněk PROKOP a Florian HOLLFELDER. Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets. \textit{Analytical Chemistry}. 2015, roč.~87, č.~1, s.~624-632. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/ac503336g.

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