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@proceedings{1214593,
author = {Mrázková, Jana and Pokorná, Martina and Wimmerová, Michaela},
booktitle = {XVI. Setkání biochemiků a molekulárních biologů},
keywords = {lectins; PA-IIL; Pseudomonas aeruginosa; targeted-random mutagenesis; high-throughput screening; mutant library},
language = {eng},
isbn = {978-80-210-7340-1},
title = {Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library},
year = {2014}
}
TY - CONF
ID - 1214593
AU - Mrázková, Jana - Pokorná, Martina - Wimmerová, Michaela
PY - 2014
TI - Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library
SN - 9788021073401
KW - lectins
KW - PA-IIL
KW - Pseudomonas aeruginosa
KW - targeted-random mutagenesis
KW - high-throughput screening
KW - mutant library
N2 - PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity.
ER -
MRÁZKOVÁ, Jana, Martina POKORNÁ a Michaela WIMMEROVÁ. Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library. In \textit{XVI. Setkání biochemiků a molekulárních biologů}. 2014. ISBN~978-80-210-7340-1.
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