Acinetobacter bohemicus sp nov widespread in natural soil and
water ecosystems in the Czech Republic

J 2014

Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic

KŘÍŽOVÁ, Lenka, Martina MAIXNEROVÁ, Ondrej ŠEDO a Alexandr NEMEC

Základní údaje

Originální název

Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic

Autoři

KŘÍŽOVÁ, Lenka (203 Česká republika), Martina MAIXNEROVÁ (203 Česká republika), Ondrej ŠEDO (203 Česká republika, garant, domácí) a Alexandr NEMEC (203 Česká republika)

Vydání

Systematic and Applied Microbiology, Jena (Germany), Urban & Fischer, 2014, 0723-2020

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.283

Kód RIV

RIV/00216224:14740/14:00074264

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.syapm.2014.07.001

UT WoS

000343635900001

Klíčová slova anglicky

rpoB; gyrB; 16S rRNA gene; ANI; Carbon source assimilation; MALDI-TOF MS

Štítky

kontrola MP, rivok

Změněno: 11. 3. 2015 11:57, Martina Prášilová

Anotace

V originále

We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (<= 77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate L-histidine and the inability to grow at 35 degrees C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T) = CCUG 63842(T) = CCM 8462(T)).

Návaznosti

GA13-26693S, projekt VaV

Název: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.

Investor: Grantová agentura ČR, Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech

Citovat

KŘÍŽOVÁ, Lenka, Martina MAIXNEROVÁ, Ondrej ŠEDO a Alexandr NEMEC. Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic. Systematic and Applied Microbiology. Jena (Germany): Urban & Fischer, 2014, roč. 37, č. 7, s. 467-473. ISSN 0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2014.07.001.

@article{1214919,
   author = {Křížová, Lenka and Maixnerová, Martina and Šedo, Ondrej and Nemec, Alexandr},
   article_location = {Jena (Germany)},
   article_number = {7},
   doi = {http://dx.doi.org/10.1016/j.syapm.2014.07.001},
   keywords = {rpoB; gyrB; 16S rRNA gene; ANI; Carbon source assimilation; MALDI-TOF MS},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic},
   url = {http://ac.els-cdn.com/S0723202014001015/1-s2.0-S0723202014001015-main.pdf?_tid=3acb56e0-c7dd-11e4-9ce5-00000aab0f01&acdnat=1426071551_9208211a79d54dbbf3b9f209899d12f7},
   volume = {37},
   year = {2014}
}

TY  - JOUR
ID  - 1214919
AU  - Křížová, Lenka - Maixnerová, Martina - Šedo, Ondrej - Nemec, Alexandr
PY  - 2014
TI  - Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic
JF  - Systematic and Applied Microbiology
VL  - 37
IS  - 7
SP  - 467-473
EP  - 467-473
PB  - Urban & Fischer
SN  - 07232020
KW  - rpoB
KW  - gyrB
KW  - 16S rRNA gene
KW  - ANI
KW  - Carbon source assimilation
KW  - MALDI-TOF MS
UR  - http://ac.els-cdn.com/S0723202014001015/1-s2.0-S0723202014001015-main.pdf?_tid=3acb56e0-c7dd-11e4-9ce5-00000aab0f01&acdnat=1426071551_9208211a79d54dbbf3b9f209899d12f7
L2  - http://ac.els-cdn.com/S0723202014001015/1-s2.0-S0723202014001015-main.pdf?_tid=3acb56e0-c7dd-11e4-9ce5-00000aab0f01&acdnat=1426071551_9208211a79d54dbbf3b9f209899d12f7
N2  - We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (<= 77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate L-histidine and the inability to grow at 35 degrees C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T) = CCUG 63842(T) = CCM 8462(T)).
ER  -

KŘÍŽOVÁ, Lenka, Martina MAIXNEROVÁ, Ondrej ŠEDO a Alexandr NEMEC. Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic. \textit{Systematic and Applied Microbiology}. Jena (Germany): Urban \&{} Fischer, 2014, roč.~37, č.~7, s.~467-473. ISSN~0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2014.07.001.

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