| BABINSKÝ, Martin, Radovan FIALA, Iva KEJNOVSKÁ, Klára BEDNÁŘOVÁ, Radek MAREK, Janos SAGI, Vladimír SKLENÁŘ a Michaela VORLÍČKOVÁ. Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding. Nucleic Acids Research. UK: Oxford University Press, 2014, roč. 42, č. 22, s. 14031-14041. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gku1245. |
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@article{1215331,
author = {Babinský, Martin and Fiala, Radovan and Kejnovská, Iva and Bednářová, Klára and Marek, Radek and Sagi, Janos and Sklenář, Vladimír and Vorlíčková, Michaela},
article_location = {UK},
article_number = {22},
doi = {http://dx.doi.org/10.1093/nar/gku1245},
keywords = {Quadruplex DNA; abasic lesion; NMR; CD;},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding},
url = {http://dx.doi.org/10.1093/nar/gku1245},
volume = {42},
year = {2014}
}
TY - JOUR
ID - 1215331
AU - Babinský, Martin - Fiala, Radovan - Kejnovská, Iva - Bednářová, Klára - Marek, Radek - Sagi, Janos - Sklenář, Vladimír - Vorlíčková, Michaela
PY - 2014
TI - Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding
JF - Nucleic Acids Research
VL - 42
IS - 22
SP - 14031-14041
EP - 14031-14041
PB - Oxford University Press
SN - 03051048
KW - Quadruplex DNA
KW - abasic lesion
KW - NMR
KW - CD;
UR - http://dx.doi.org/10.1093/nar/gku1245
L2 - http://dx.doi.org/10.1093/nar/gku1245
N2 - Abasic (AP) lesions are the most frequent type of damages occurring in cellular DNA. Here we describe the conformational effects of AP sites substituted for 2'-deoxyadenosine in the first (ap7), second (ap13) or third (ap19) loop of the quadruplex formed in K+ by the human telomere DNA 5'-d[AG3(TTAG3)3]. CD spectra and electrophoresis reveal that the presence of AP sites does not hinder the formation of intramolecular quadruplexes. NMR spectra show that the structural heterogeneity is substantially reduced in ap7 and ap19 as compared to that in the wild-type. These two (ap7 and ap19) sequences are shown to adopt the hybrid-1 and hybrid-2 quadruplex topology, respectively, with AP site located in a propeller-like loop. All three studied sequences transform easily into parallel quadruplex in dehydrating ethanol solution. Thus, the AP site in any loop region facilitates the formation of the propeller loop. Substitution of all adenines by AP sites stabilizes the parallel quadruplex even in the absence of ethanol. Whereas guanines are the major determinants of quadruplex stability, the presence or absence of loop adenines substantially influences quadruplex folding. The naturally occurring adenine-lacking sites in the human telomere DNA can change the quadruplex topology in vivo with potentially vital biological consequences.
ER -
BABINSKÝ, Martin, Radovan FIALA, Iva KEJNOVSKÁ, Klára BEDNÁŘOVÁ, Radek MAREK, Janos SAGI, Vladimír SKLENÁŘ a Michaela VORLÍČKOVÁ. Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding. \textit{Nucleic Acids Research}. UK: Oxford University Press, 2014, roč.~42, č.~22, s.~14031-14041. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gku1245.
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