2014
Identification of key regulatory metabolites in hESCs by MALDI-TOF-MS
SALYKIN, Anton, Filippo AMATO, Petr DVOŘÁK, Vladimír ROTREKL, Josef HAVEL et. al.Základní údaje
Originální název
Identification of key regulatory metabolites in hESCs by MALDI-TOF-MS
Autoři
SALYKIN, Anton (643 Rusko, domácí), Filippo AMATO (380 Itálie, domácí), Petr DVOŘÁK (203 Česká republika, domácí), Vladimír ROTREKL (203 Česká republika, domácí) a Josef HAVEL (203 Česká republika, garant, domácí)
Vydání
ESAS 2014, 2014
Další údaje
Jazyk
angličtina
Typ výsledku
Prezentace na konferencích
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/14:00074304
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
regulatory metabolites; MALDI-TOF-MS; mass spectrometry
Klíčová slova anglicky
regulatory metabolites; MALDI-TOF-MS; mass spectrometry
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 28. 4. 2015 09:30, Ing. Andrea Mikešková
Anotace
V originále
The ability of human embryonic stem cells (hESC) to differentiate into any cell type of human body presents a tool for regenerative medicine raising the need for advanced cultivation and differentiation protocols. Metabolic homeostasis in stem cells is shown to be different from that in somatic cells. To meet their energetic demands stem cells rely mostly on glycolysis rather than on oxidative phosphorylation. Energy metabolism contributes to molecular mechanisms controlling stem cell identity, selfrenewal and differentiation. In this context, clear understanding of how cell fate decisions and metabolic processes are intertwined is essential for identification of key regulatory nodes capable of differential stem cell fate choice regulation. Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) has been proved to be a powerful tool to study proteomic and metabolic fingerprints of both bacterial and mammalian cell lines. The technique with optimized stem cell extraction protocol allowed for identification of more than 20 individual metabolites contributing to the dynamic changes in human pluripotent stem cell metabolism. By employing advanced analysis we were able to identify the key metabolites that contribute to the maximum changes in the stem cell metabolism upon varying physiological conditions. Our data shed light on the plasticity of metabolic homeostasis in human pluripotent stem cells during cultivation in vitro opening the space for development of more specific cultivation protocols for translational medicine.
Návaznosti
| ED2.1.00/03.0086, projekt VaV |
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| GA13-19910S, projekt VaV |
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