Identification of AHK2- and AHK3-like cytokinin receptors in
Brassica napus reveals two subfamilies of AHK2 orthologues

J 2015

Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues

KUDEROVÁ, Alena; Lucia GALLOVÁ; Katarína KURICOVÁ; Eliška NEJEDLÁ; Anna ČURDOVÁ et al.

Základní údaje

Originální název

Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues

Autoři

KUDEROVÁ, Alena; Lucia GALLOVÁ; Katarína KURICOVÁ; Eliška NEJEDLÁ; Anna ČURDOVÁ; Lenka MICENKOVÁ; Ondřej PLÍHAL; David ŠMAJS; Lukáš SPÍCHAL a Jan HEJÁTKO

Vydání

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2015, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.677

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/15:00080639

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

CHASE-containing His kinase; E. coli-based live-cell competitive receptor assay; gene structure; JBnB library; modular protein architecture; phylogenetic analysis

Štítky

EL OK, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 8. 2015 10:44, Martina Prášilová

Anotace

V originále

Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1–BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1–BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1–3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.20.0043, projekt VaV

Název: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

EE2.3.20.0189, projekt VaV

Název: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

GAP305/11/0756, projekt VaV

Název: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Akronym: HKRD-AHP)

Investor: Grantová agentura ČR, Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis

GA13-25280S, projekt VaV

Název: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Grantová agentura ČR, Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

GA13-39982S, projekt VaV

Název: Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese.

Investor: Grantová agentura ČR, Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese

Přiložené soubory

EL_1217495.pdf

Požádat o autorskou verzi souboru

Citovat

KUDEROVÁ, Alena; Lucia GALLOVÁ; Katarína KURICOVÁ; Eliška NEJEDLÁ; Anna ČURDOVÁ; Lenka MICENKOVÁ; Ondřej PLÍHAL; David ŠMAJS; Lukáš SPÍCHAL a Jan HEJÁTKO. Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2015, roč. 66, č. 1, s. 339-353. ISSN 0022-0957. Dostupné z: https://doi.org/10.1093/jxb/eru422.

@article{1217495,
   author = {Kuderová, Alena and Gallová, Lucia and Kuricová, Katarína and Nejedlá, Eliška and Čurdová, Anna and Micenková, Lenka and Plíhal, Ondřej and Šmajs, David and Spíchal, Lukáš and Hejátko, Jan},
   article_location = {Oxford},
   article_number = {1},
   doi = {https://doi.org/10.1093/jxb/eru422},
   keywords = {CHASE-containing His kinase; E. coli-based live-cell competitive receptor assay; gene structure; JBnB library; modular protein architecture; phylogenetic analysis},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues},
   url = {http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf},
   volume = {66},
   year = {2015}
}

TY  - JOUR
ID  - 1217495
AU  - Kuderová, Alena - Gallová, Lucia - Kuricová, Katarína - Nejedlá, Eliška - Čurdová, Anna - Micenková, Lenka - Plíhal, Ondřej - Šmajs, David - Spíchal, Lukáš - Hejátko, Jan
PY  - 2015
TI  - Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues
JF  - Journal of Experimental Botany
VL  - 66
IS  - 1
SP  - 339-353
EP  - 339-353
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - CHASE-containing His kinase
KW  - E. coli-based live-cell competitive receptor assay
KW  - gene structure
KW  - JBnB library
KW  - modular protein architecture
KW  - phylogenetic analysis
UR  - http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf
L2  - http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf
N2  - Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1–BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1–BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1–3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.
ER  -

KUDEROVÁ, Alena; Lucia GALLOVÁ; Katarína KURICOVÁ; Eliška NEJEDLÁ; Anna ČURDOVÁ; Lenka MICENKOVÁ; Ondřej PLÍHAL; David ŠMAJS; Lukáš SPÍCHAL a Jan HEJÁTKO. Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2015, roč.~66, č.~1, s.~339-353. ISSN~0022-0957. Dostupné z: https://doi.org/10.1093/jxb/eru422.

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