2015
Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues
KUDEROVÁ, Alena, Lucia GALLOVÁ, Katarína KURICOVÁ, Eliška NEJEDLÁ, Anna ČURDOVÁ et. al.Základní údaje
Originální název
Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues
Autoři
KUDEROVÁ, Alena (203 Česká republika, domácí), Lucia GALLOVÁ (203 Česká republika), Katarína KURICOVÁ (703 Slovensko, domácí), Eliška NEJEDLÁ (203 Česká republika, domácí), Anna ČURDOVÁ (703 Slovensko, domácí), Lenka MICENKOVÁ (703 Slovensko, domácí), Ondřej PLÍHAL (203 Česká republika), David ŠMAJS (203 Česká republika, domácí), Lukáš SPÍCHAL (203 Česká republika) a Jan HEJÁTKO (203 Česká republika, garant, domácí)
Vydání
Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2015, 0022-0957
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 5.677
Kód RIV
RIV/00216224:14740/15:00080639
Organizační jednotka
Středoevropský technologický institut
UT WoS
000351660900028
Klíčová slova anglicky
CHASE-containing His kinase; E. coli-based live-cell competitive receptor assay; gene structure; JBnB library; modular protein architecture; phylogenetic analysis
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 19. 8. 2015 10:44, Martina Prášilová
Anotace
V originále
Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1–BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1–BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1–3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.
Návaznosti
ED1.1.00/02.0068, projekt VaV |
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EE2.3.20.0043, projekt VaV |
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EE2.3.20.0189, projekt VaV |
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GAP305/11/0756, projekt VaV |
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GA13-25280S, projekt VaV |
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GA13-39982S, projekt VaV |
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