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@article{1217495,
author = {Kuderová, Alena and Gallová, Lucia and Kuricová, Katarína and Nejedlá, Eliška and Čurdová, Anna and Micenková, Lenka and Plíhal, Ondřej and Šmajs, David and Spíchal, Lukáš and Hejátko, Jan},
article_location = {Oxford},
article_number = {1},
doi = {http://dx.doi.org/10.1093/jxb/eru422},
keywords = {CHASE-containing His kinase; E. coli-based live-cell competitive receptor assay; gene structure; JBnB library; modular protein architecture; phylogenetic analysis},
language = {eng},
issn = {0022-0957},
journal = {Journal of Experimental Botany},
title = {Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues},
url = {http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf},
volume = {66},
year = {2015}
}
TY - JOUR
ID - 1217495
AU - Kuderová, Alena - Gallová, Lucia - Kuricová, Katarína - Nejedlá, Eliška - Čurdová, Anna - Micenková, Lenka - Plíhal, Ondřej - Šmajs, David - Spíchal, Lukáš - Hejátko, Jan
PY - 2015
TI - Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues
JF - Journal of Experimental Botany
VL - 66
IS - 1
SP - 339-353
EP - 339-353
PB - OXFORD UNIV PRESS
SN - 00220957
KW - CHASE-containing His kinase
KW - E. coli-based live-cell competitive receptor assay
KW - gene structure
KW - JBnB library
KW - modular protein architecture
KW - phylogenetic analysis
UR - http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf
L2 - http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf
N2 - Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1–BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1–BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1–3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.
ER -
KUDEROVÁ, Alena, Lucia GALLOVÁ, Katarína KURICOVÁ, Eliška NEJEDLÁ, Anna ČURDOVÁ, Lenka MICENKOVÁ, Ondřej PLÍHAL, David ŠMAJS, Lukáš SPÍCHAL a Jan HEJÁTKO. Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2015, roč.~66, č.~1, s.~339-353. ISSN~0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/eru422.
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