Inflammatory Profiling of Schwann Cells in Contact with Growing
Axons Distal to Nerve Injury

J 2014

Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana HRADILOVÁ SVÍŽENSKÁ

Základní údaje

Originální název

Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

Autoři

DUBOVÝ, Petr (203 Česká republika, garant, domácí), Ilona KLUSÁKOVÁ (203 Česká republika, domácí) a Ivana HRADILOVÁ SVÍŽENSKÁ (203 Česká republika, domácí)

Vydání

BioMed Research International, USA, Hindawi Publishing Corporation, 2014, 2314-6133

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.579

Kód RIV

RIV/00216224:14740/14:00078951

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1155/2014/691041

UT WoS

000335836400001

Klíčová slova anglicky

activated Schwann cell; growing axons; reinnervation; inflammatory mediators

Štítky

kontrola MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 4. 2015 09:09, Martina Prášilová

Anotace

V originále

Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

286154, interní kód MU

Název: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Akronym: SYLICA)

Investor: Evropská unie, SYLICA - Synergies of Life and Material Sciences to Create a New Future, Kapacity

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana HRADILOVÁ SVÍŽENSKÁ. Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury. BioMed Research International. USA: Hindawi Publishing Corporation, 2014, roč. 2014, April, s. "nestránkováno", 7 s. ISSN 2314-6133. Dostupné z: https://dx.doi.org/10.1155/2014/691041.

@article{1220228,
   author = {Dubový, Petr and Klusáková, Ilona and Hradilová Svíženská, Ivana},
   article_location = {USA},
   article_number = {April},
   doi = {http://dx.doi.org/10.1155/2014/691041},
   keywords = {activated Schwann cell; growing axons; reinnervation; inflammatory mediators},
   language = {eng},
   issn = {2314-6133},
   journal = {BioMed Research International},
   title = {Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury},
   url = {http://www.hindawi.com/journals/bmri/2014/691041/},
   volume = {2014},
   year = {2014}
}

TY  - JOUR
ID  - 1220228
AU  - Dubový, Petr - Klusáková, Ilona - Hradilová Svíženská, Ivana
PY  - 2014
TI  - Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury
JF  - BioMed Research International
VL  - 2014
IS  - April
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - Hindawi Publishing Corporation
SN  - 23146133
KW  - activated Schwann cell
KW  - growing axons
KW  - reinnervation
KW  - inflammatory mediators
UR  - http://www.hindawi.com/journals/bmri/2014/691041/
L2  - http://www.hindawi.com/journals/bmri/2014/691041/
N2  - Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana HRADILOVÁ SVÍŽENSKÁ. Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury. \textit{BioMed Research International}. USA: Hindawi Publishing Corporation, 2014, roč.~2014, April, s.~''nestránkováno'', 7 s. ISSN~2314-6133. Dostupné z: https://dx.doi.org/10.1155/2014/691041.

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