The ATRA-induced differentiation of medulloblastoma cells is
enhanced with LOX/COX inhibitors: an analysis of gene
expression.

J 2014

The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.

CHLAPEK, Petr, Jakub NERADIL, Martina LOJOVÁ, Karel ZITTERBART, Jaroslav ŠTĚRBA et. al.

Základní údaje

Originální název

The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.

Autoři

CHLAPEK, Petr (203 Česká republika, domácí), Jakub NERADIL (203 Česká republika, domácí), Martina LOJOVÁ (203 Česká republika, domácí), Karel ZITTERBART (203 Česká republika, domácí), Jaroslav ŠTĚRBA (203 Česká republika, domácí) a Renata VESELSKÁ (203 Česká republika, garant, domácí)

Vydání

Cancer Cell International, 2014, 1475-2867

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.766

Kód RIV

RIV/00216224:14310/14:00079004

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1186/1475-2867-14-51

UT WoS

000338146500001

Klíčová slova anglicky

all-trans retinoic acid; caffeic acid; celecoxib; medullobastoma; LOX and COX inhibitors

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 11. 2015 22:11, prof. RNDr. Renata Veselská, Ph.D., M.Sc.

Anotace

V originále

A detailed analysis of the expression of 440 cancer-related genes was performed after the combined treatment of medulloblastoma cells with all-trans retinoic acid (ATRA) and inhibitors of lipoxygenases (LOX) and cyclooxygenases (COX). The Daoy and D283 Med medulloblastoma cell lines were chosen for this study. Caffeic acid (an inhibitor of 5-LOX) and celecoxib (an inhibitor on COX-2) were used in combined treatment with ATRA. The results clearly confirmed that the differentiating effects of ATRA should be enhanced via its combined administration with caffeic acid or celecoxib. An increased expression of the genes that encoded the proteins participating in induced differentiation and cytoskeleton remodeling was detected in both cell lines in a concentration-dependent manner. This effect was also observed for the CDKN1A gene encoding the p21 protein, which is an important regulator of the cell cycle, and for the genes encoding proteins that are associated with proteasome activity. Furthermore, our results showed that D283 Med cells are significantly more sensitive to treatment with ATRA alone than Daoy cells.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

NR9341, projekt VaV

Název: Modulace antineoplastického efektu retinoidů LOX/COX inhibitory na modelu dětských solidních nádorů: in vitro studie

Investor: Ministerstvo zdravotnictví ČR, Modulace antineoplastického efektu retinoidů LOX/COX inhibitory na modelu dětských solidních nádorů: in vitro studie

Citovat

CHLAPEK, Petr, Jakub NERADIL, Martina LOJOVÁ, Karel ZITTERBART, Jaroslav ŠTĚRBA a Renata VESELSKÁ. The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression. Cancer Cell International. 2014, roč. 14, June, s. "nestránkováno", 10 s. ISSN 1475-2867. Dostupné z: https://dx.doi.org/10.1186/1475-2867-14-51.

@article{1225452,
   author = {Chlapek, Petr and Neradil, Jakub and Lojová, Martina and Zitterbart, Karel and Štěrba, Jaroslav and Veselská, Renata},
   article_number = {June},
   doi = {http://dx.doi.org/10.1186/1475-2867-14-51},
   keywords = {all-trans retinoic acid; caffeic acid; celecoxib; medullobastoma; LOX and COX inhibitors},
   language = {eng},
   issn = {1475-2867},
   journal = {Cancer Cell International},
   title = {The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.},
   url = {http://www.cancerci.com/content/14/1/51},
   volume = {14},
   year = {2014}
}

TY  - JOUR
ID  - 1225452
AU  - Chlapek, Petr - Neradil, Jakub - Lojová, Martina - Zitterbart, Karel - Štěrba, Jaroslav - Veselská, Renata
PY  - 2014
TI  - The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.
JF  - Cancer Cell International
VL  - 14
IS  - June
SP  - "nestránkováno"
EP  - "nestránkováno"
SN  - 14752867
KW  - all-trans retinoic acid
KW  - caffeic acid
KW  - celecoxib
KW  - medullobastoma
KW  - LOX and COX inhibitors
UR  - http://www.cancerci.com/content/14/1/51
L2  - http://www.cancerci.com/content/14/1/51
N2  - A detailed analysis of the expression of 440 cancer-related genes was performed after the combined treatment of medulloblastoma cells with all-trans retinoic acid (ATRA) and inhibitors of lipoxygenases (LOX) and cyclooxygenases (COX). The Daoy and D283 Med medulloblastoma cell lines were chosen for this study. Caffeic acid (an inhibitor of 5-LOX) and celecoxib (an inhibitor on COX-2) were used in combined treatment with ATRA. The results clearly confirmed that the differentiating effects of ATRA should be enhanced via its combined administration with caffeic acid or celecoxib. An increased expression of the genes that encoded the proteins participating in induced differentiation and cytoskeleton remodeling was detected in both cell lines in a concentration-dependent manner. This effect was also observed for the CDKN1A gene encoding the p21 protein, which is an important regulator of the cell cycle, and for the genes encoding proteins that are associated with proteasome activity. Furthermore, our results showed that D283 Med cells are significantly more sensitive to treatment with ATRA alone than Daoy cells.
ER  -

CHLAPEK, Petr, Jakub NERADIL, Martina LOJOVÁ, Karel ZITTERBART, Jaroslav ŠTĚRBA a Renata VESELSKÁ. The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression. \textit{Cancer Cell International}. 2014, roč.~14, June, s.~''nestránkováno'', 10 s. ISSN~1475-2867. Dostupné z: https://dx.doi.org/10.1186/1475-2867-14-51.

Podrobný výpis o publikaci