Simultaneous determination of reduced and oxidized glutathione
in tissues by a novel liquid chromatography-mass spectrometry
method: application in an inhalation study of Cd nanoparticles

J 2014

Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles

BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Jana LEBEDOVÁ, Luděk BLÁHA, Z. VECERA et. al.

Základní údaje

Originální název

Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles

Autoři

BLÁHOVÁ, Lucie (203 Česká republika, garant, domácí), Jiří KOHOUTEK (203 Česká republika, domácí), Jana LEBEDOVÁ (203 Česká republika, domácí), Luděk BLÁHA (203 Česká republika, domácí), Z. VECERA (203 Česká republika), M. BUCHTOVA (203 Česká republika), Ivan MÍŠEK (203 Česká republika) a Klára HILSCHEROVÁ (203 Česká republika, domácí)

Vydání

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2014, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.436

Kód RIV

RIV/00216224:14310/14:00079120

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00216-014-8033-z

UT WoS

000341834500013

Klíčová slova anglicky

Glutathione; LC MS/MS; Tissues; Oxidative stress; Nanoparticle; Cadmium oxide

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 4. 2015 08:19, Mgr. Lucie Bláhová, Ph.D.

Anotace

V originále

The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. The performance of the method was further demonstrated in a model experiment addressing changes in GSH and GSSG concentrations in lung of mice exposed to CdO nanoparticles during acute 72 h and chronic 13-week exposures. Inhalation exposure led to increased GSH concentrations in lung. GSSG levels were in general not affected; nonsignificant suppression occurred only after the longer 13-week period of exposure. The developed method for the sensitive detection of both GSH and GSSG in very low tissue mass enables these parameters to be studied in cases where only a little sample is available, i.e. in small organisms or in small amounts of tissue.

Návaznosti

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Jana LEBEDOVÁ, Luděk BLÁHA, Z. VECERA, M. BUCHTOVA, Ivan MÍŠEK a Klára HILSCHEROVÁ. Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2014, roč. 406, č. 24, s. 5867-5876. ISSN 1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-014-8033-z.

@article{1226667,
   author = {Bláhová, Lucie and Kohoutek, Jiří and Lebedová, Jana and Bláha, Luděk and Vecera, Z. and Buchtova, M. and Míšek, Ivan and Hilscherová, Klára},
   article_location = {HEIDELBERG},
   article_number = {24},
   doi = {http://dx.doi.org/10.1007/s00216-014-8033-z},
   keywords = {Glutathione; LC MS/MS; Tissues; Oxidative stress; Nanoparticle; Cadmium oxide},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles},
   volume = {406},
   year = {2014}
}

TY  - JOUR
ID  - 1226667
AU  - Bláhová, Lucie - Kohoutek, Jiří - Lebedová, Jana - Bláha, Luděk - Vecera, Z. - Buchtova, M. - Míšek, Ivan - Hilscherová, Klára
PY  - 2014
TI  - Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles
JF  - Analytical and Bioanalytical chemistry
VL  - 406
IS  - 24
SP  - 5867-5876
EP  - 5867-5876
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Glutathione
KW  - LC MS/MS
KW  - Tissues
KW  - Oxidative stress
KW  - Nanoparticle
KW  - Cadmium oxide
N2  - The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. The performance of the method was further demonstrated in a model experiment addressing changes in GSH and GSSG concentrations in lung of mice exposed to CdO nanoparticles during acute 72 h and chronic 13-week exposures. Inhalation exposure led to increased GSH concentrations in lung. GSSG levels were in general not affected; nonsignificant suppression occurred only after the longer 13-week period of exposure. The developed method for the sensitive detection of both GSH and GSSG in very low tissue mass enables these parameters to be studied in cases where only a little sample is available, i.e. in small organisms or in small amounts of tissue.
ER  -

BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Jana LEBEDOVÁ, Luděk BLÁHA, Z. VECERA, M. BUCHTOVA, Ivan MÍŠEK a Klára HILSCHEROVÁ. Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2014, roč.~406, č.~24, s.~5867-5876. ISSN~1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-014-8033-z.

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