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@article{1226667, author = {Bláhová, Lucie and Kohoutek, Jiří and Lebedová, Jana and Bláha, Luděk and Vecera, Z. and Buchtova, M. and Míšek, Ivan and Hilscherová, Klára}, article_location = {HEIDELBERG}, article_number = {24}, doi = {http://dx.doi.org/10.1007/s00216-014-8033-z}, keywords = {Glutathione; LC MS/MS; Tissues; Oxidative stress; Nanoparticle; Cadmium oxide}, language = {eng}, issn = {1618-2642}, journal = {Analytical and Bioanalytical chemistry}, title = {Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles}, volume = {406}, year = {2014} }
TY - JOUR ID - 1226667 AU - Bláhová, Lucie - Kohoutek, Jiří - Lebedová, Jana - Bláha, Luděk - Vecera, Z. - Buchtova, M. - Míšek, Ivan - Hilscherová, Klára PY - 2014 TI - Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles JF - Analytical and Bioanalytical chemistry VL - 406 IS - 24 SP - 5867-5876 EP - 5867-5876 PB - SPRINGER HEIDELBERG SN - 16182642 KW - Glutathione KW - LC MS/MS KW - Tissues KW - Oxidative stress KW - Nanoparticle KW - Cadmium oxide N2 - The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. The performance of the method was further demonstrated in a model experiment addressing changes in GSH and GSSG concentrations in lung of mice exposed to CdO nanoparticles during acute 72 h and chronic 13-week exposures. Inhalation exposure led to increased GSH concentrations in lung. GSSG levels were in general not affected; nonsignificant suppression occurred only after the longer 13-week period of exposure. The developed method for the sensitive detection of both GSH and GSSG in very low tissue mass enables these parameters to be studied in cases where only a little sample is available, i.e. in small organisms or in small amounts of tissue. ER -
BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Jana LEBEDOVÁ, Luděk BLÁHA, Z. VECERA, M. BUCHTOVA, Ivan MÍŠEK a Klára HILSCHEROVÁ. Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2014, roč.~406, č.~24, s.~5867-5876. ISSN~1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-014-8033-z.
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